Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii  

作　　者：Han Wang Sai Ma Alfred Mugambi Mariga Qiuhui Hu Qian Xu Anxiang Su Ning Ma Gaoxing Ma 

机构地区：[1]College of Food Science and Engineering,Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing 210023,China [2]School of Agriculture and Food Science,Meru University of Science and Technology,Meru 972-60200,Kenya

出　　处：《Food Science and Human Wellness》2024年第5期3031-3042,共12页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(31901623);Major Public Welfare Projects in Henan Province(201300110200);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Natural polysaccharides named PEP-0.1-1,PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study.Results showed that molecular weights of these polysaccharides were 3235,2041 and 23933 Da,respectively.Further,structural characterization revealed that PEP-0.1-1 had a→4-α-D-Glcp-1→backbone and contained→4)-α-D-Glcp and→4)-β-D-Glcp reducing end groups.PEP-0-1 backbone contained→4-α-D-Glcp-1→and→6-α-3-O-Me-D-Galp-1→,and the side chains containedα-D-Glcp,β-D-Manp-1→andα-D-Glcp-3→.However,PEP-0-2 backbone consisted of→4-α-DGlcp-1→and→6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→while the side chains containedα-D-Glcp andβ-D-Manp-1→.Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4,nitric oxide(NO),tumor necrosis factor-α,interleukin(IL)-1βand IL-6 in lipopolysaccharide(LPS)-induced inflammation of RAW264.7 cells,as well as the over secretion of the above cell cytokines.Moreover,Western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of c-Jun N-terminal kinases protein induced by LPS in mitogen activated protein kinase pathway,along with the relieving on the inhibition effect of LPS on IκB-αprotein expression.In summary,the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with antiinflammation activities.

关 键 词：Pleurotus eryngii POLYSACCHARIDES Structural characterization Anti-inflammatory activity 

分 类 号：TS201.2[轻工技术与工程—食品科学]