鸭源肠致病性大肠杆菌的耐药特征及全基因组学分析  

Antimicrobial resistance analysis and genomic characteristics of enteropathogenic Escherichia coli derived from ducks

作　　者：李俊霖 胡家萌 汪骆 李佳芮 刘淏天 夏静[1,2] 崔敏 邹立扣 韩新锋 LI Jun-lin;HU Jia-meng;WANG Luo;LI Jia-rui;LIU Hao-tian;XIA Jing;CUI Min;ZOU Li-kou;HAN Xin-feng(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Animal Diseases and Human Health of Sichuan Province,Chengdu 611130,China;College of Resources,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,成都611130 [2]动物疫病与人类健康四川省重点实验室,成都611130 [3]四川农业大学资源学院,成都611130

出　　处：《中国人兽共患病学报》2024年第8期701-707,共7页Chinese Journal of Zoonoses

基　　金：四川省自然科学基金(No.2023NSFSC0175);四川省重大科技专项(No.2022ZDZX0017)联合资助。

摘　　要：目的了解鸭源肠致病性大肠杆菌(EPEC)的耐药情况及全基因组特征,探究鸭源EPEC的致病潜力,为临床治疗提供理论基础。方法PCR鉴定出EPEC分离株,采用微量肉汤稀释法进行药敏试验,并对其进行全基因组测序,分析EPEC的血清型、ST型、质粒不相容群类型及其携带的耐药基因与毒力基因。结果共鉴定出10株EPEC,血清型包括O71∶H40和O3∶H21两种;所有EPEC菌株均表现出多重耐药,对环丙沙星、链霉素、四环素、多粘菌素B最为耐药,耐药率高达100%,对头孢西丁最为敏感,对阿米卡星、亚胺培南较敏感;所有菌株均携带有四环素耐药基因tet(A),以及超广谱β-内酰胺酶(ESBL)耐药基因,包括bla OXA-10、bla TEM-1A、bla TEM-1B;分离株检测到多种毒力基因,与分泌系统相关的毒力基因最多,未检测到bfp基因及perABC基因。结论鸭源EPEC分离株都为非典型EPEC(aEPEC),具有多重耐药性,检测到多种质粒不相容群,携带多种耐药基因与毒力基因,对人具有潜在致病性,需要加强对鸭源EPEC的监测以有效控制鸭源EPEC的传播,防止其对人类健康造成危害。Enteropathogenic Escherichia coli(EPEC),a zoonotic foodborne pathogen,can induce severe and prolonged diarrhea,thus substantially affecting global public health safety.To understand the pathogenicity of EPEC and its potential risk to human health,this study investigated the antimicrobial resistance and genome-wide characteristics of EPEC originating from ducks.After identification of EPEC with the plate method and PCR,antimicrobial susceptibility of the isolates was examined with the microbroth dilution method.In addition,analyses of serotype,sequence type(ST),and plasmid incompatibility groups were conducted with whole-genome sequencing(WGS)and bioinformatic methods.Ten EPEC isolates were identified,including serotypes O71∶H40 and O3∶H21.All EPEC strains exhibited multiple drug resistance.The highest proportion of resistance(100%)was observed to ciprofloxacin,streptomycin,tetracycline,and polymyxin B.In contrast,the isolates showed susceptibility to cefoxitin,amikacin,and imipenem.Furthermore,all strains carried the tetracycline resistance gene tet(A)and extended-spectrumβ-lactamase(ESBL)resistance genes,including bla OXA-10,bla TEM-1A,and bla TEM-1B.Various virulence genes,associated primarily with the secretory system,were detected in the isolates.However,no bfp genes or perABC genes were identified,thus indicating that the EPEC isolates were atypical EPEC(aEPEC).The results demonstrated the presence of multiple antimicrobial resistance,multiple resistance and virulence genes,and various plasmid incompatibility groups,thus indicating potential pathogenicity to humans.Strengthened monitoring of duck-derived EPEC is crucial to effectively control the spread of the pathogen and safeguard public health.

关 键 词：肠致病性大肠杆菌 耐药性 全基因组测序 耐药基因 毒力基因 

分 类 号：R378.2[医药卫生—病原生物学]