上海市首次自腹泻患者中检出格隆柏沙门菌的表型和分子特征研究  

Phenotypic and molecular characteristics of a Salmonella Grumpensis isolate from a patient with diarrhea in Shanghai,China

作　　者：王闻卿 黄卫春[3] 苏靖华 尤舒琪 郑英杰[4] 杨博雯 黄红[1] 郝莉鹏 许学斌[5] WANG Wen-qing;HUANG Wei-chun;SU Jing-hua;YOU Shu-qi;ZHENG Ying-jie;YANG Bo-wen;HUANG Hong;HAO Li-peng;XU Xue-bin(Department of Microbiology of Pudong New Area Center for Disease Control and Prevention,Shanghai 200136,China;Fudan University Pudong Institute of Preventive Medicine,Shanghai 200136,China;Department of Laboratory Medicine,Shanghai Children’s Medical Center,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China;School of Public Health,Fudan University,Shanghai 200032,China;Division of Pathogen Testing and Analysis,Shanghai Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市浦东新区疾病预防控制中心微生物检验科,上海200136 [2]复旦大学浦东预防医学研究院,上海200136 [3]上海交通大学医学院附属上海儿童医学中心检验科,上海200127 [4]复旦大学公共卫生学院,上海200032 [5]上海市疾病预防控制中心病原生物检定所,上海200336

出　　处：《中国人兽共患病学报》2024年第8期732-738,共7页Chinese Journal of Zoonoses

基　　金：上海市浦东新区卫生健康公共卫生高原学科项目(No.PWYggy2021-01)资助。

摘　　要：目的研究上海市首次自腹泻患者中检出格隆柏沙门菌的表型和分子特征,为沙门菌病防控提供依据。方法对格隆柏分离株(2023JD76)进行生化鉴定、血清凝集试验、抗菌药物敏感性试验以及全基因组测序(whole genome sequencing,WGS)。收集全球格隆柏沙门菌全基因组序列,进行血清分型、多位点序列分型(multilocus sequence typing,MLST)、耐药基因和毒力基因预测,分析2023JD76与全球株的系统进化关系。结果2023JD76血清分型符合抗原式(Salmonella Grumpensis,13,23:d:1,7),对20种抗菌药物均敏感,MLST型预测为2060(ST2060),携带氨基糖甙类药物耐药基因aac(6′)-Iaa和5类毒力基因,包括黏附基因csg、rat,分泌和转运基因sip、inv,伤寒毒素基因cdt、plt,侵袭性基因营养代谢因子mgt和抗菌肽抗性因子mig。36株全球格隆柏菌株共携带10种耐药基因,不同毒力基因携带率为58.33%~100%。进化树将全球格隆柏沙门菌基因组分为2个支系,支系Ⅰ以ST751(88.89%,16/18)为主,支系Ⅱ以ST2060(89.47%,17/19)为主。本次分离菌株2023JD76处于支系Ⅱ内,其序列型与全球人源粪便株和人源脑脊液株全基因组序列密切相关。结论本研究首次分离国内腹泻患者中的格隆柏沙门菌,不同地理来源菌株间耐药表型差异大,应加强监测以防范全球潜在的传播风险。This study was aimed at studying the phenotypic and molecular characteristics of a Salmonella Grumpensis isolate from a patient with diarrhea in Shanghai,to provide evidence for the prevention of salmonellosis.Biochemical identification,serum agglutination testing,antimicrobial susceptibility testing,and whole genome sequencing(WGS)were performed on isolate 2023JD76.Global Salmonella Grumpensis genome sequences were searched and downloaded for serotyping prediction,multilocus sequence typing(MLST),prediction of antimicrobia resistance genes and virulence genes,and phylogenetic analysis of 2023JD76.The 2023JD76 strain was identified as Salmonella Grumpensis(13,23:d:1,7)with ST2060,and was susceptible to 20 antimicrobial agents.Strain 2023JD76 carried the aminoglycoside resistance gene aac(6′)-Iaa and five types of virulence genes:the adhesion genes csg and rat;the secretion and transport genes sip and inv;the typhoid toxin genes cdt and plt;the invasive gene nutrient metabolism factor mgt;and the antimicrobial peptide resistance factor mig.Global S.Grumpensis strains harbored ten types of antimicrobial resistance genes whose prevalence ranged from 58.33%to 100%.The global genome sequences of S.Grumpensis were divided into two lineages.Lineage I was dominated by ST751(88.89%,16/18),and lineage II was dominated by ST2060(89.47%,17/19).The genome sequence of strain 2023JD76 belonged to lineage II,and was closely related to the genome sequences from human fecal and human cerebrospinal fluid.This study provides the first report of a S.Grumpensis isolate from the stool of a patient with diarrhea in China.Considerable variability in antimicrobial resistance genes was observed among genome sequences from different sources,and the strains harbored a substantial number of virulence genes.Enhanced surveillance should be emphasized to prevent a potential risk of global dissemination.

关 键 词：格隆柏沙门菌 全基因组测序 多位点序列分型 耐药基因 毒力基因 

分 类 号：R378.2[医药卫生—病原生物学]