Credible and Facile Fluorometric Detection of Soybean Trypsin Inhibitor Activity with a Water-Soluble Poly(diphenylacetylene) Derivative  

作　　者：Jiao Tang Xiao Tang Jiaxiang Tang Wei Qi Qianwei Pan Jinhong Zeng Housheng Xia Jianping Zhou Zhongyi Sheng Junfeng Niu 

机构地区：[1]Zhejiang Tiantai Xianghe Industrial Co.,Ltd.,Taizhou 317200,China [2]Zhejiang Xianghe Track Fastener Research Institute,Taizhou 317200,China [3]Shaoxing Institute for Food and Drug Control,Shaoxing 312000,China [4]Key Laboratory of Chemical and Biological Processing Technology for Farm Products of Zhejiang Province,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,China

出　　处：《Transactions of Tianjin University》2024年第4期305-313,共9页天津大学学报（英文版）

基　　金：The authors appreciate the support from the Zhe-jiang Province Lingyan Key R&D Project(No.2022C01177);the Zhejiang Administration for Market Regulation Eyas Program Cultiva-tion Project(No.CY2022355).

摘　　要：Herein,a novel method for fl uorometric detection of soybean trypsin inhibitor(SBTI)activity based on a water-soluble poly(diphenylacetylene)derivative was reported.Fluorescence quenching of the polymer via p-nitroaniline,produced from the trypsin-catalyzed decomposition of N-benzoyl-DL-arginine-4-nitroanilide hydrochloride(L-BAPA),was well described using the Stern-Volmer equation.SBTI activity was quantitatively assessed based on changes in the fl uorescence intensity of the polymer.This strategy has several advantages,such as high sensitivity and ease of operation.Moreover,its applicability to other biochemical analyses is promising.

关 键 词：Fluorometric detection Soybean trypsin inhibitor Inhibition activity Fluorescence quenching 

分 类 号：TS201.21[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]