季也蒙迈耶氏酵母提高再造烟叶浓缩液香气品质研究  

Improving the Aroma Quality of Reconstituted Tobacco Concentrate by Meyerozyma guilliermondii

作　　者：武士杰 张鹏[3] 于晓梅 信琪 付秋娟[3] 杜咏梅[3] 刘洪斌[1] 高丽伟 WU Shijie;ZHANG Peng;YU Xiaomei;XIN Qi;FU Qiujuan;DU Yongmei;LIU Hongbin;GAO Liwei(Tianjin University of Science&Technology,Tianjin 300222,China;Yunnan Tobacco Reconstiuted Tobacco Company Limited,Kunming 650106,China;Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Qingdao Agricultural University,Qingdao 266109,China;Qilu University of Technology,Jinan 250353,China)

机构地区：[1]天津科技大学,天津300222 [2]云南中烟再造烟叶有限责任公司,昆明650106 [3]中国农业科学院烟草研究所,青岛266101 [4]青岛农业大学,青岛266109 [5]齐鲁工业大学,济南250353

出　　处：《中国烟草科学》2024年第4期102-110,120,共10页Chinese Tobacco Science

基　　金：云南中烟再造烟叶有限责任公司科技项目(GYCX2021-2);云南中烟工业有限责任公司重点科技项目(2022YL03);中国烟草总公司重点研发项目(110202102018);中国烟草总公司重大科技项目(110202201005 JY-05)。

摘　　要：为提升再造烟叶的香气质,从葡萄表面筛选到一株能够发酵再造烟叶浓缩液进行产香的微生物Y-7,对其进行了菌种鉴定和发酵条件优化,并检测了其对再造烟叶的增香效果。结果表明,Y-7菌株为季也蒙迈耶氏酵母(Meyerozyma guilliermondii),最佳生长条件为pH 7.0、25℃。使用该酵母对60%再造烟叶浓缩液发酵24 h,能显著提升浓缩液中多种挥发性致香物质的含量,其中酮类、醇类、酸类、酚类和其他致香物质分别提升26.92%、101.07%、349.47%、33.62%和20.35%;新增致香物质11种。Y-7发酵浓缩液能够显著提升再造烟叶的香气质和香气量、甜感和余味舒适性,改善其品质。综上,季也蒙迈耶氏酵母Y-7在塑造再造烟叶浓缩液香气品质方面具有良好的应用前景。To improve the aroma quality of reconstituted tobacco,a microorganism strain Y-7 capable of fermenting and producing aroma from reconstituted tobacco concentrate was screened from grape surfaces.The strain was molecularly identified,and the fermentation conditions were optimized.Also,its aroma enhancement effect on reconstituted tobacco was evaluated.Results indicated that the strain Y-7 was Meyerozyma guilliermondii,and the optimal growth conditions were 25°C and pH 7.0.Fermentation of 60%concentrated tobacco extract with this yeast for 24 hours significantly increased the content of various volatile aroma compounds.Specifically,ketones,alcohols,acids,phenols,and other compounds with aroma displayed increases of 26.92%,101.07%,349.47%,33.62%,and 20.35%,respectively.Additionally,eleven novel aroma compounds were generated.Y-7fermentation of concentrated extract notably enhanced the aroma quality and quantity,sweetness,and after taste of reconstituted tobacco,thereby improving its overall quality.In conclusion,Meyerozyma guilliermondii Y-7 shows promising application prospects for shaping the aroma quality of reconstituted tobacco concentrate.

关 键 词：季也蒙迈耶氏酵母 再造烟叶浓缩液 致香成分 

分 类 号：TS452[农业科学—烟草工业]