16S rDNA鉴定腐败椰果罐头中的微生物  

Identification of Microorganisms in Spoiled Coconut Cans by 16S rDNA

作　　者：宋美玲 郭三保[2] 廖莉[1] 吴晴阳 黄胜和 SONG Meiling;GUO Sanbao;LIAO Li;WU Qingyang;HUANG Shenghe(Department of Basic Medicine,Jiangxi College of Traditional Chinese Medicine,Fuzhou 344000,China;Department of Pharmacy,Jiangxi College of Traditional Chinese Medicine,Fuzhou 344000,China)

机构地区：[1]江西中医药高等专科学校医学基础部,江西抚州344000 [2]江西中医药高等专科学校药学系,江西抚州344000

出　　处：《食品安全导刊》2024年第24期71-74,共4页China Food Safety Magazine

基　　金：江西省抚州市社会发展指导性科技计划项目(抚科社字[2023]7号序列号3)。

摘　　要：以腐败椰果罐头为试材,选用4种培养基分离纯化腐败椰果罐头中的细菌,扩增细菌16S rDNA,对16S rDNA序列进行同源比对,并构建系统进化树,鉴定细菌种属。结果显示,从腐败椰果罐头中分离得到38株分离菌,经16S rDNA分子生物学方法鉴定为Paenibacillus humicus、Staphylococcus warneri、Sphingomonas sp.和Liquorilactobacillus satsumensis。Taking spoiled coconut cans as test material,four kinds of culture media were selected to separate and purify bacteria in spoiled coconut cans,amplify bacterial 16S rDNA,compare the 16S rDNA sequences,construct a phylogenetic tree,and identify the bacterial species.The results showed that 38 isolates were obtained from spoiled coconut cans and identified by 16S rDNA molecular biology method as Paenibacillus humicus,Staphylococcus warneri,Sphingomonas sp.,Liquorilactobacillus satsumensis.

关 键 词：腐败椰果罐头 16S rDNA 细菌 鉴定 

分 类 号：TS295.6[轻工技术与工程—农产品加工及贮藏工程]