ICE1 interacts with IDD14 to transcriptionally activate QQS to increase pollen germination and viability  

作　　者：Landi Luo Yan Zheng Xieshengyang Li Qian Chen Danni Yang Zhijia Gu Ya Yang Yunqiang Yang Xiangxiang Kong Yongping Yang 

机构地区：[1]CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Xishuangbanna 666303,China [2]Germplasm Bank of Wild Species,Yunnan Key Laboratory for Crop Wild Relatives Omics,Kunming Institute of Botany,The Chinese Academy of Sciences,Kunming 650201,China [3]School of Agriculture,Yunnan University,Kunming 650091,China [4]Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,The Chinese Academy of Sciences,Kunming 650201,China

出　　处：《Journal of Integrative Plant Biology》2024年第8期1801-1819,共19页植物学报（英文版）

基　　金：supported by the National Science Foundation of China(Grant Nos.32170385,32200306,and 31500221);the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(Grant No.2019QZKK0502)。

摘　　要：In flowering plants,sexual reproductive success depends on the production of viable pollen grains.However,the mechanisms by which QUA QUINE STARCH(QQS)regulates pollen development and how transcriptional activators facilitate the transcription of QQS in this process remain poorly understood.Here,we demonstrate that INDUCER OF CBF EXPRESSION 1(ICE1),a basic helix–loop–helix(b HLH)transcription factor,acts as a key transcriptional activator and positively regulates QQS expression to increase pollen germination and viability in Arabidopsis thaliana by interacting with INDETERMINATE DOMAIN14(IDD14).In our genetic and biochemical experiments,overexpression of ICE1 greatly promoted both the activation of QQS and high pollen viability mediated by QQS.IDD14 additively enhanced ICE1 function by promoting the binding of ICE1 to the QQS promoter.In addition,mutation of ICE1 significantly repressed QQS expression;the impaired function of QQS and the abnormal anther dehiscence jointly affected pollen development of the ice1-2 mutant.Our results also showed that the enhancement of pollen activity by ICE1 depends on QQS.Furthermore,QQS interacted with CUT1,the key enzyme for long-chain lipid biosynthesis.This interaction both promoted CUT1 activity and regulated pollen lipid metabolism,ultimately determining pollen hydration and fertility.Our results not only provide new insights into the key function of QQS in promoting pollen development by regulating pollen lipid metabolism,but also elucidate the mechanism that facilitates the transcription of QQS in this vital developmental process.

关 键 词：TRANSCRIPTIONAL METABOLISM jointly 

分 类 号：Q944[生物学—植物学]