小槐花叶黄酮碳苷类成分的分离与结构鉴定  

Isolation and structural identification of C-glycosyl flavonoids from the leaves of Ohwia caudata(Thunberg)H.Ohashi

作　　者：张倩倩 杨丁山 石中唯 阮静雅 王涛[1,2] 张祎[1,2] ZHANG Qianqian;YANG Dingshan;SHI Zhongwei;RUAN Jingya;WANG Tao;ZHANG Yi(Tianjin Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,天津市现代中药重点实验室,天津301617 [2]天津中医药大学,组分中药国家重点实验室,天津301617

出　　处：《沈阳药科大学学报》2024年第9期1180-1189,共10页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金面上项目(82074118)。

摘　　要：目的对小槐花(Ohwia caudata(Thunberg)H.Ohashi)叶的化学成分进行研究,丰富其物质基础。方法采用加热回流提取法对小槐花干燥叶进行提取,综合利用多种色谱法对小槐花叶的化学成分进行分离纯化,采用核磁共振波谱和质谱技术对其进行结构鉴定。结果从小槐花干燥叶体积分数70%乙醇提取物中分离鉴定了异牡荆苷(1)、牡荆苷(2)、芹菜素-6,8-二-C-β-D-吡喃葡萄糖苷(3)、异肥皂草苷(4)、异牡荆素-2″-O-鼠李糖苷(5)、当药黄素(6)、异当药黄素(7)、2″-O-鼠李糖基当药黄素(8)、2″-O-鼠李糖基异当药黄素(9)、异荭草素(10)、荭草素(11)、异荭草素2″-O-鼠李糖苷(12)、日当药黄素(13)、2″-O-鼠李糖基日当药黄素(14)、异金雀花素-7-O-葡萄糖苷(15)等15个黄酮碳苷类成分。结论化合物4、5、7、11-15为首次从小槐花属植物中分离得到。Objective The chemical constituent′s investigation of Ohwia caudata(Thunberg)H.Ohashi leaves was carried out to enrich its material basis.Methods The dried leaves of O.caudata were extracted by heating reflux to obtain 70%EtOH extraction.Then,the extraction was separated and purified by various chromatographic methods to gain compounds,whose structures were identified by using NMR and mass spectra mainly.Results Fifteen C-glycosyl flavonoids named as isovitexin(1),vitexin(2),6,8-di-C-β-D-glucopyranosylapigenin(3),isosaponarin(4),isovitexin-2″-O-rhamnoside(5),swertisin(6),isoswertisin(7),2″-O-rhamnosylswertisin(8),2″-α-rhamnopyranosyl-7-O-methylvitexin(9),luteolin 6-C-β-glucopyranoside(10),luteolin 8-C-β-glucopyranoside(11),isoorientin-2″-O-rhamnoside(12),swertiajaponin(13),2″-O-rhamnopyranosyl-7-O-methylisoorientin(14),and isoscoparin-7-O-glucoside(15)were obtained from the 70%ethanol extract of O.caudata leaves.Conclusion Among them,compounds 4,5,7,11-15 are gained from Ohwia genus for the first time.

关 键 词：小槐花叶 黄酮碳苷 结构鉴定 阻旋异构体 

分 类 号：R914[医药卫生—药物化学]