外周血IMA、CBX7 mRNA、miRNA-181c-5p对缺血性脑卒中患者预后不良的预测价值  

Predictive value of IMA,CBX7 mRNA and miRNA-181c-5p in peripheral blood for the poor prognosis of patients with ischemic stroke

作　　者：郭晓凯 刘侠[2] 陈会霞 郭庆浩 GUO Xiaokai;LIU Xia;CHEN Huixia;GUO Qinghao(Department of Clinical Laboratory,Handan Hospital of Traditional Chinese Medicine,Handan,Hebei 056000,China;Department of Encephalopathy,Handan Hospital of Traditional Chinese Medicine,Handan,Hebei 056000,China)

机构地区：[1]河北省邯郸市中医院检验科,河北邯郸056000 [2]河北省邯郸市中医院脑病科,河北邯郸056000

出　　处：《检验医学与临床》2024年第18期2652-2656,共5页Laboratory Medicine and Clinic

基　　金：河北省医学科学研究基金项目(20210356)。

摘　　要：目的分析外周血缺血修饰蛋白(IMA)、色素框同源蛋白7(CBX7)信使RNA(mRNA)、微小RNA(miRNA)-181c-5p对缺血性脑卒中(IS)患者预后不良的预测价值。方法选取2021年1-12月在河北省邯郸市中医院就诊的150例IS患者作为观察组,根据改良Rankin量表(mRS)评分将IS患者分为预后良好组(mRS评分≤2分)和预后不良组(mRS评分>2分),另选取同期在河北省邯郸市中医院体检的150例健康人群作为对照组。比较观察组、对照组外周血IMA、CBX7 mRNA、miRNA-181c-5p水平,比较预后良好组和预后不良组临床资料。采用Pearson相关分析IS患者外周血IMA、CBX7 mRNA、miRNA-181c-5p水平与mRS评分的相关性;采用多因素Logistic回归分析IS患者预后不良的危险因素;绘制受试者工作特征(ROC)曲线分析外周血IMA、CBX7 mRNA、miRNA-181c-5p对IS患者预后不良的预测价值。结果观察组外周血IMA、CBX7 mRNA、miRNA-181c-5p水平明显高于对照组,差异均有统计学意义(P<0.05)。随访结果显示预后良好组有103例患者,预后不良组有47例患者。预后良好组和预后不良组性别、年龄,以及合并高血压、糖尿病患者比例比较,差异均无统计学意义(P>0.05)。预后不良组外周血IMA、CBX7 mRNA、miRNA-181c-5p水平高于预后良好组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,外周血IMA、CBX7 mRNA、miRNA-181c-5p水平升高为IS患者预后不良的危险因素(P<0.05)。Pearson相关分析结果显示,IS患者外周血IMA、CBX7 mRNA、miRNA-181c-5p水平与mRS评分呈正相关(r=0.629、0.475、0.419,P<0.05)。ROC曲线分析结果显示,外周血IMA、CBX7 mRNA、miRNA-181c-5p 3项指标联合预测IS患者预后不良的曲线下面积(AUC)为0.873,高于单项指标预测的AUC(Z=3.347、3.125、2.974,P<0.05)。结论外周血IMA、CBX7 mRNA、miRNA-181c-5p水平升高为IS患者预后不良的危险因素,与其预后存在相关性,3项指标联合检测对IS患者预后不良有较高Objective To analyze the predictive value of peripheral blood ischemic modified protein(IMA),pigment-frame homologous protein 7(CBX7)messenger RNA(mRNA)and microRNA(miRNA)-181c-5p in peripheral blood for the poor prognosis of patients with ischemic stroke(IS).Methods A total of 150 IS patients who were treated in Handan Hospital of Traditional Chinese Medicine from January to December 2021 were selected as the observation group.According to the modified Rankin Scale(mRS)score,the IS patients were divided into the good prognosis group(mRS score≤2)and the poor prognosis group(mRS score>2).Another 150 healthy people who underwent physical examination in Handan Hospital of Traditional Chinese Medicine were selected as the control group.Peripheral IMA,CBX7 mRNA and miRNA-181c-5p levels in observation group and control group were compared,and clinical data of the good prognosis group and the poor prognosis group were compared.Pearson correlation was used to analyze the correlation between peripheral IMA,CBX7 mRNA,miRNA-181c-5p levels and mRS scores in IS patients.Multivariate Logistic regression was used to analyze the risk factors of poor prognosis in patients with IS.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of peripheral IMA,CBX7 mRNA and miRNA-181c-5p for poor prognosis in patients with IS.Results Peripheral IMA,CBX7 mRNA and miRNA-181c-5p levels in observation group were significantly higher than those in control group,with statistical significance(P<0.05).The follow-up results showed that there were 103 patients in the good prognosis group and 47 patients in the poor prognosis group.There was no significant difference in gender,age,the proportion of patients with hypertension or diabetes between the good prognosis group and the poor prognosis group(P>0.05).Peripheral IMA,CBX7 mRNA and miRNA-181c-5p levels in the poor prognosis group were higher than those in the good prognosis group,and the differences were statistically significant(P<0.05).Multivariate Logistic regre

关 键 词：缺血性脑卒中 预后 缺血修饰蛋白 色素框同源蛋白 微小RNA-181c-5p 相关性 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R446.112[医药卫生—临床医学]