Lugol's iodine增强Micro-CT成像评估口腔鳞状细胞癌切缘状态的研究  

Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma

作　　者：郁佳鑫 刘鲲宇 章茜[1] 蒲玉梅[2] 胡勤刚[1] 夏成万 王育新[1] YU Jiaxin;LIU Kunyu;ZHANG Qian;PU Yumei;HU Qingang;XIA Chengwan;WANG Yuxin(Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China;Department of Oral and Maxillofacial Head and Neck Oncology Surgery,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院,南京市口腔医院口腔颌面创伤正颌整形外科,南京大学口腔医学研究所,江苏南京210008 [2]南京大学医学院附属口腔医院,南京市口腔医院口腔颌面头颈肿瘤外科,南京大学口腔医学研究所,江苏南京210008

出　　处：《口腔医学研究》2024年第9期797-802,共6页Journal of Oral Science Research

基　　金：江苏省重点研发计划项目(社会发展)(编号:BE2021609);南京市卫生科技发展项目(编号:YKK22177)。

摘　　要：目的:本研究拟使用Lugol's iodine增强Micro-CT成像三维评估口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)切缘状态。方法:本研究共收集68例人OSCC组织样本,所有样本经3%Lugol's iodine染色12 h后行Micro-CT扫描(层厚为50μm)。随后将经Lugol's iodine染色后的样品行常规苏木精-伊红(hematoxylin-eosin,HE)染色切片(厚度为4μm),制作数字化病理切片。分别测量影像图像和病理图像的黏膜切缘(mucosal margin,MM)和深部切缘(deep margin,DM)。结果:Lugol's iodine增强Micro-CT成像可以在三维空间中清晰区分肿瘤边界,且Micro-CT成像相较于二维病理切片,可以更准确地测量MM和DM,发现阳性切缘及临界切缘。结论:Lugol's iodine增强Micro-CT成像可以全面评估OSCC切缘,提供更全面、更清晰的肿瘤边缘信息,指导临床治疗方案选择和预后评估。Objective:To assess the incisal margin status of oral squamous cell carcinoma(OSCC)in three dimensions using Lugol's iodine enhanced Micro-CT imaging.Methods:A total of 68 human OSCC in vitro samples were collected for this study.All samples were stained with 3%solution of Lugol's iodine for 12 hours and underwent Micro-CT scanning with a layer thickness of 50μm.Subsequently,the Lugol's iodine stained samples were subjected to routine hematoxylin-eosin(HE)staining sections(4μm)to create digital pathological sections.The mucosal margin(MM)and deep margin(DM)were measured separately on both the imaging and pathological images.Results:Lugol's iodine enhanced Micro-CT imaging can effectively differentiate tumor boundaries in three-dimensional space,providing more accurate measurements of MM and DM compared to two-dimensional pathological sections,thus enabling identification of positive incisal margins and critical incisal margins.Conclusion:Lugol's iodine enhanced Micro-CT imaging allows comprehensive evaluation of OSCC incisal margins in three dimensions,offering more comprehensive and clear information regarding tumor margins which can guide clinical treatment plan selection and prognosis assessment.

关 键 词：口腔鳞状细胞癌 显微计算机断层成像 卢戈氏碘液 三维 

分 类 号：R739.8[医药卫生—肿瘤]