Huoxue Tongjiang decoction-resisted reflux esophagitis by activation stem cell factor/c-kit/interstitial cell of cajal pathway and regulating the T-helper 17/regulatory T-cells balance in rats  

作　　者：Yan Liu Yan-Ping Tang Pei-Cai Li Lei Liu Xi Liu Si-Yu Liu Lei Yang 

机构地区：[1]Division of Gastroenterology,Hospital of Integrated Chinese and Western Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300000,China [2]Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair,Hospital of Integrated Chinese and Western Medicine,Tianjin 300000,China [3]Institute of Integrative Medicine for Acute Abdominal Diseases,Hospital of Integrated Chinese and Western Medicine,Tianjin 300000,China

出　　处：《Traditional Medicine Research》2024年第12期1-12,共12页TMR传统医学研究

基　　金：supported by the National Natural Science Foundation of China(No.81573737 and 82074213);the science foundation of Tianjin Municipal Health Bureau(No.2023169 and 2021045);the Tianjin Municipal Health Commission Science and Technology Project(No.TJWJ2022QN057).

摘　　要：Background:Huoxue Tongjiang decoction(HXTJD)is an effective prescription for treating reflux esophagitis(RE).We investigated the effects of HXTJD on esophageal motility and mucosal inflammation in a rat RE model.Methods:Chemical composition of HXTJD was analyzed by ultrahigh-performance liquid chromatography Q-Orbitrap mass spectrometry(MS).The change rates of mean contraction tension forces,mean amplitudes,and mean frequencies for the lower esophageal sphincter(LES)were recorded using the isolated tissue bath system,mechanical tension transducer,and PowerLab physiological recorder.After weighing the stomach,the phenol red labeling method was used to measure the gastric emptying rate.The LES ultrastructure was observed through transmission electron microscopy.Immunofluorescence and western blotting were used to detect the number of interstitial cells of Cajal(ICC)and the expression levels of c-kit protein,connexin43(Cx43),and stem cell factor(SCF).Flow cytometric analysis and enzyme-linked immunosorbent assay were conducted to detect the percentages of T helper 17(Th17)cells and regulatory T(Treg)cells and the serum concentrations of interleukin 6(IL-6),interleukin 17(IL-17),and interleukin 10(IL-10)in the rats.Results:We identified 28 chemical constituents in HXTJD.Regarding esophageal motility,we revealed that HXTJD increased the mean contraction tension forces,mean amplitudes,and mean frequency change rate of LES and the gastric emptying rate;decreased stomach weight;and improved the LES ultrastructure.Additionally,HXTJD increased the number of ICC-positive cells,and c-kit,Cx43,and SCF expression levels.Regarding esophageal inflammation,HXTJD significantly decreased the percentage of Th17 cells,and IL-6 and IL-17 concentrations,and increased the percentage of Treg cells and IL-10 concentration.Conclusion:HXTJD was found to be efficacious in the rat RE model.It may promote esophageal motility and alleviate the inflammatory response by activating the SCF/c-kit/ICC pathway and regulating the Th17/Treg cell balanc

关 键 词：Huoxue Tongjiang decoction reflux esophagitis SCF/c-kit/ICC pathway Th17/Treg cell esophageal motility mucosal inflammation 

分 类 号：R571[医药卫生—消化系统]