circ_0007059靶向miR-1303对前列腺癌LNCaP细胞恶性生物学行为的影响  

Effect of circ_0007059 targeting miR-1303 on malignant biological behavior of LNCaP cells in prostate cancer

作　　者：王魏龙 田峰[1] 赵修民[1] 彭强[1] 徐容[2] Wang Weilong;Tian Feng;Zhao Xiumin;Peng Qiang;Xu Rong(Department of Urology,Mianyang People's Hospital,Mianyang 053000,Sichuan,China;Department of Oncology,Mianyang People's Hospital,Mianyang 053000,Sichuan,China)

机构地区：[1]绵阳市人民医院泌尿外科,四川绵阳053000 [2]绵阳市人民医院肿瘤内科,四川绵阳053000

出　　处：《中国男科学杂志》2024年第4期48-53,共6页Chinese Journal of Andrology

基　　金：绵阳市卫生健康委员会医学科研项目(202235)。

摘　　要：目的探讨circ_0007059对前列腺癌LNCaP细胞恶性生物学行为的影响。方法选取2017年5月至2020年在我院就诊的41例前列腺癌患者的癌组织和癌旁组织标本;体外培养前列腺癌LNCaP细胞,采用质粒转染分别过表达/敲低circ_0007059表达、抑制miR-1303表达,并同时上调circ_0007059和miR-1303表达。转染后,检测circ_0007059和miR-1303表达、细胞增殖、迁移、侵袭,以及Ki-67、MMP-2、MMP-9蛋白表达,并验证circ_0007059和miR-1303的靶向关系。结果前列腺癌组织中circ_0007059表达水平降低,miR-1303表达水平升高(P<0.05)。circ_0007059过表达或抑制miR-1303表达后,LNCaP细胞活性、迁移和侵袭能力,以及Ki-67、MMP-2、MMP-9蛋白水平均降低。circ_0007059靶向调控miR-1303;上调miR-1303逆转了circ_0007059过表达对LNCaP细胞的作用。结论过表达circ_0007059可抑制LNCaP细胞的恶性生物学行为,其机制可能与靶向下调miR-1303有关。Objective To investigate the effect of circ_0007059 on malignant biological behavior of prostate cancer LNCaP cells.Methods The cancer tissue and paracancerous tissue specimens of 41 prostate cancer patients in our hospital from May 2017 to 2020 were selected.In vitro culture of prostate cancer LNCaP cells,plasmid transfection was used to overexpress/knockdown circ_0007059 expression,inhibit miR-1303 expression,and up-regulate circ_0007059 and miR-1303 expression,respectively.After transfection,circ_0007059 and miR-1303 expression,cell proliferation,migration,invasion,Ki-67,MMP-2,MMP-9 protein expression were detected,and the targeting relationship between circ_0007059 and miR-1303 was verified.Results The expression of circ_0007059 in prostate cancer tissue was decreased,and the expression of miR-1303 was increased(P<0.05).After circ_0007059 overexpression or inhibition of miR-1303 expression,the LNCaP cells viability,migration and invasion ability and Ki-67,MMP-2 and MMP-9 protein levels was decreased.circ_0007059 targets and regulates miR-1303;up-regulation of miR-1303 reversed the effects of circ_0007059 overexpression on LNCaP cells.Conclusions Overexpression of circ_0007059 can inhibit the malignant biological behavior of LNCaP cells,and the mechanism may be related to the targeted down-regulation of miR-1303.

关 键 词：RNA 环状 微RNAS 前列腺肿瘤 增殖 迁移 

分 类 号：R737.25[医药卫生—肿瘤]