Sodium Leak Channel in Glutamatergic Neurons of the Lateral Parabrachial Nucleus Helps to Maintain Respiratory Frequency Under Sevoflurane Anesthesia  

作　　者：Lin Wu Donghang Zhang Yujie Wu Jin Liu Jingyao Jiang Cheng Zhou 

机构地区：[1]Department of Anesthesiology,West China Hospital,Sichuan University,Chengdu 610041,China [2]Laboratory of Anesthesia and Critical Care Medicine,National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology,West China Hospital,Sichuan University,Chengdu 610041,China

出　　处：《Neuroscience Bulletin》2024年第8期1127-1140,共14页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(82271290 and 82301403);the Natural Science Foundation of Sichuan Province(2022NSFSC1399);the Health Commission of Sichuan Province(21PJ014);the Key R&D(Major Science and Technology Project)of the Sichuan Science and Technology Department(2023YFS0138 and 2023ZYD0168).

摘　　要：The lateral parabrachial nucleus(PBL)is implicated in the regulation of respiratory activity.Sodium leak channel(NALCN)mutations disrupt the respiratory rhythm and influence anesthetic sensitivity in both rodents and humans.Here,we investigated whether the NALCN in PBL glutamatergic neurons maintains respiratory function under general anesthesia.Our results showed that chemogenetic activation of PBL glutamatergic neurons increased the respiratory frequency(RF)in mice;whereas chemogenetic inhibition suppressed RF.NALCN knockdown in PBL glutamatergic neurons but not GABAergic neurons significantly reduced RF under physiological conditions and caused more respiratory suppression under sevoflurane anesthesia.NALCN knockdown in PBL glutamatergic neurons did not further exacerbate the respiratory suppression induced by propofol or morphine.Under sevoflurane anesthesia,painful stimuli rapidly increased the RF,which was not affected by NALCN knockdown in PBL glutamatergic neurons.This study suggested that the NALCN is a key ion channel in PBL glutamatergic neurons that maintains respiratory frequency under volatile anesthetic sevoflurane but not intravenous anesthetic propofol.

关 键 词：Glutamatergic neurons Lateral parabrachial nucleus NALCN RESPIRATION General anesthesia 

分 类 号：R741[医药卫生—神经病学与精神病学]