膳食纤维食物摄入与虚弱症的因果关系——一项两样本孟德尔随机化研究  

Causal relationship between fiber food intake and frailty:a two-sample Mendelian randomization study

作　　者：李建兴 王卓彥 LI Jian-xing;WANG Zhuo-yan(School of Public Health,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510310,China)

机构地区：[1]广东药科大学公共卫生学院,广东广州510310

出　　处：《现代预防医学》2024年第17期3128-3133,共6页Modern Preventive Medicine

摘　　要：目的本研究采用两样本孟德尔随机化分析富含膳食纤维的谷物、水果、蔬菜三种食物摄入与虚弱症之间的因果关系。方法本研究从IEU数据库获取全基因组关联研究(GWAS)数据,纳入的单核苷酸多态性(SNP)位点数据均来自欧洲人群且不存在样本重叠。所选取的工具变量均满足三条重要假设,将谷物、水果、蔬菜摄入作为暴露因素,虚弱症作为结局因素。采用逆方差加权法(IVW)作为主要分析方法,并运用MR-Egger法、加权中位数法、简单模式法、加权模式法对结果进行验证和准确性评估,同时使用多种敏感性分析方法检测异质性和水平多效性。结果共提取了谷物摄入的31个SNPs、水果摄入的46个SNPs、蔬菜摄入的13个SNPs作为工具变量并纳入研究分析。IVW法显示谷物摄入(OR=0.756,95%CI:0.679~0.841,P<0.001)、水果摄入(OR=0.881,95%CI:0.777~0.999,P=0.048)是虚弱症发生风险的保护因素,蔬菜摄入(OR=1.201,95%CI:0.960~1.501,P=0.109)与虚弱症发生风险不存在因果关系,敏感性分析以上结果均稳健。结论谷物摄入、水果摄入与虚弱症发生风险呈负向因果关系,蔬菜摄入与虚弱症发生风险不存在因果关系。Objective To investigate the causal relationship of the intake of fiber-rich foods,grains,fruits and vegetables with frailty through two-sample Mendelian randomization analysis.Methods We obtained genome-wide association study(GWAS)data from the IEU database,including single nucleotide polymorphism(SNP)data from the European population without sample overlap.The selected instrumental variables satisfied three key assumptions,with grain,fruit,and vegetable intake considered as exposure factors and frailty as the outcome factor.The inverse variance weighted(IVW)method was used as the primary analysis method,complemented by MR-Egger,weighted median,simple mode,and weighted mode methods for result validation and accuracy assessment.Additionally,various sensitivity analysis methods were employed to detect heterogeneity and horizontal pleiotropy.Results A total of 31 SNPs for grain intake,46 SNPs for fruit intake,and 13 SNPs for vegetable intake were extracted as instrumental variables for analysis.The IVW method indicated that grain intake(OR=0.756,95%CI:0.679-0.841,P<0.001)and fruit intake(OR=0.881,95%CI:0.777-0.999,P=0.048)were protective factors against the risk of frailty,while vegetable intake(OR=1.201,95%CI:0.960-1.501,P=0.109)showed no causal relationship with frailty risk.Sensitivity analyses confirmed the robustness of these results.Conclusion There is a negative causal relationship between grain and fruit intake and the risk of frailty,whereas vegetable intake does not have a causal relationship with frailty risk.

关 键 词：膳食纤维食物 虚弱症 因果关系 孟德尔随机化 

分 类 号：R151.4[医药卫生—营养与食品卫生学]