α-淀粉酶水解马铃薯中淀粉提取还原糖参数优化  

Optimization of Reducing Sugar Parameters for Starch Extraction Fromα-amylase Hydrolyzed Potato

作　　者：吴海龙 白圆 Wu Hailong;Bai Yuan(Department of Municipal and Environment Engineering,Lanzhou Jiaotong University,Lanzhou 730070,Gansu,China;Key Laboratory of Yellow River Water Environment in Gansu Province,Lanzhou 730070,Gansu,China)

机构地区：[1]兰州交通大学环境与市政工程学院,甘肃兰州730070 [2]甘肃省水环境重点实验室,甘肃兰州730070

出　　处：《绿色科技》2024年第14期247-253,共7页Journal of Green Science and Technology

基　　金：甘肃省自然基金项目(编号:21JR11RA068);甘肃省高校产业支持计划项目资金(编号2020c-38)。

摘　　要：采用单因素实验方法及响应面法对α-淀粉酶水解马铃薯中的淀粉的酶用量、pH值、温度及时间等条件进行了优化。通过响应面法得出最佳组合条件为pH=6.0,酶加量37 mg(0.37%),酶解时间5.1 h,酶解温度66℃。在此工艺条件下马铃薯水解程度最大达到,还原糖提取率为5.104%与还原糖提取率预测值5.20%误差低于2%。酶解动力学试验结果显示,在不同的底物浓度条件下,酶解速率的变化规律符合米氏方程的特征。采用双倒数作图法计算出K m=99.13 g/L,V max=11.66 g/(L·h),得米氏方程式为V=2.92[S]/(99.13+[S]),R 2=0.99752,方程极显著,可很好地模拟α-淀粉酶酶解动力学过程。研究可以为资源化废弃马铃薯提供借鉴。This study was optimized by using a single factor experiment method and a response surface method to optimize the amount of amzyla amount,pH,temperature and time of the amyloid hydrolysis potato.The optimum combination conditions were obtained by the response surface method of pH 6.0,and the enzyme was added 37 mg(0.37%).The enzymatic hydrolysis time was 5.1 h,and the enzymatic solution was 66℃.In this process,the degree of hydrolysis of potato is maximized.The extraction rate of reducing sugar was 5.104%and the error between the extraction rate of reducing sugar and the predicted value of 5.20%is less than 2%.The results of enzymolysis kinetics test showed that the change of enzymolysis rate was consistent with the characteristics of Mi equation under different substrate concentrations.The K m and V max were calculated by the method of double reciprocal drawing which were 99.13 g/L and 2.92 g/(L·h)separately.The Michaelis Equation is V=2.92[S]/(99.13+[S])and R 2=0.99752.The equation was very significant which could be used to simulate the enzymolysis kinetic process ofα-amylase.The research could provide a reference for resource-based potato waste.

关 键 词：Α-淀粉酶 响应面法 酶解动力学 还原糖提取 米氏方程 

分 类 号：TS235.2[轻工技术与工程—粮食、油脂及植物蛋白工程]