人参皂苷Rg_(3)对氧化应激诱导神经细胞损伤的保护作用  

Protective Effect of Ginsenosides Rg_(3)against Nerve Cell Injury Induced by Oxidative Stress

作　　者：于子翔 何元洋 王一媚 董博奇 刘水 刘迪 冯宪敏 YU Zixiang;HE Yuanyang;WANG Yimei;DONG Boqi;LIU Shui;LIU Di;FENG Xianmin(College of Basic Medicine,Jilin Medical University,Jilin 132013,China)

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013

出　　处：《现代食品科技》2024年第8期116-122,共7页Modern Food Science and Technology

基　　金：吉林省自然科学基金项目(20200201146JC)。

摘　　要：该文探讨了人参皂苷Rg_(3)对氧化应激诱导PC12细胞损伤的保护作用。通过细胞抗氧化活性(CAA)实验检测Rg_(3)对氧自由基诱导细胞氧化的抑制作用,并建立H_(2)O_(2)诱导的PC12细胞氧化应激模型,CCK-8法检测Rg_(3)对细胞的保护和毒性作用,运用Western blot检测Rg_(3)对炎症和凋亡相关信号通路中p65磷酸化蛋白和CREB磷酸化蛋白的影响。结果发现Rg_(3)对氧自由基的抗氧化能力约是Trolox的7.3倍。200μmol/L H_(2)O_(2)诱导PC12氧化应激时细胞活力下降至60.11%,p-p65蛋白相对量为1.30,p-CREB蛋白相对量为0.80。10、20和40μmol/L Rg_(3)保护组的细胞活力分别提高至74.00%、76.04%、80.36%,呈浓度依赖式提高;细胞p-p65蛋白相对量分别降低至1.18、1.03、0.82;细胞p-CREB蛋白相对量分别提高至1.31、1.75、2.30。结果表明人参皂苷Rg_(3)可能通过清除氧自由基,参与NF-κB/CREB通路调控,减轻PC12细胞炎症和凋亡,抑制氧化应激诱导的神经细胞损伤,为人参的神经营养特性研究及相关功能食品开发提供理论依据。To determine protective effect of ginsenoside Rg_(3)on oxidative stress-induced injury in PC12 cells,the inhibitory effect of Rg_(3)on oxygen free radicals induced cell oxidation was detected in a cellular antioxidant activity experiment.To this end,an oxidative stress model of PC12 cells induced by H_(2)O_(2)was established,whose cell viability was detected using the CCK-8 method.The effects of Rg_(3)on the protein expression of p65 and CREB,related to inflammatory and apoptosis,were detected by western blotting.The results showed that the antioxidant ability of Rg_(3)in response to oxygen free radicals was approximately 7.3-fold that of Trolox.Cell viability was decreased by 60.11%after treatment with 200μmol/L H_(2)O_(2).Meanwhile,the relative expression of p-p65 protein was increased to 1.30,and p-CREB protein was decreased to 0.80.The cell viabilities of protective groups pretreated with different concentrations of Rg_(3)(10,20,and 40μmol/L)were found to increase by 74.00%,76.04%,and 80.36%,respectively.The relative expressions of p-p65 protein decreased to 1.18,1.03,and 0.82,respectively.Meanwhile,the relative expression of p-CREB protein increased to 1.31,1.75 and 2.30,respectively.These results suggest that ginsenoside Rg_(3)may protect PC12 cells from oxidative damage by scavenging oxygen free radicals.The underlying mechanisms may be related to the NF-κB/CREB pathway,which reduces inflammation and apoptosis in PC12 cells,thereby inhibiting the oxidative stress-induced nerve cell damage.These findings provide a theoretical basis for the study of the neurotrophic characteristics of ginseng and the development of related functional foods.

关 键 词：人参皂苷Rg_(3) 氧化应激 CREB NF-ΚBP65 神经损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]