厚壳贻贝mTOR信号通路关键基因在幼虫生长发育中的表达模式分析  

Analysis of expression patterns of key genes in the mTOR signaling pathway in the larval growth and development of Mytilus coruscus

作　　者：梁鑫杰 徐敏慧 叶莹莹 李继姬 Liang Xinjie;Xu Minhui;Ye yingying;Li jiji(National Engineering Research Center For Marine Aquaculture,Zhejiang Ocean University zhoushan 316022,China)

机构地区：[1]浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山316022

出　　处：《海洋学报》2024年第7期110-119,共10页

基　　金：舟山市科技计划项目(2021C21017)。

摘　　要：在水产养殖领域,对厚壳贻贝(Mytilus coruscus)的生长发育机制进行深入探讨,可为实现生长发育分子调控奠定理论基础。为了探究厚壳贻贝幼虫生长与发育差异基因的表达模式,本研究采用转录组测序和实时荧光定量PCR分析技术,对与生长特征相关的基因和分子途径的表达差异进行了初步研究。研究重点关注哺乳动物雷帕霉素靶蛋白(mammalian Target Of Rapamycin,mTOR)信号通路在厚壳贻贝幼虫生长和发育中的影响及调节作用。通过对不同发育时期(担轮幼虫期、D形幼虫期、壳顶幼虫期、眼点幼虫期和稚贝期)的基因表达模式进行分析,结果表明mTOR信号通路可能在厚壳贻贝幼虫的生长发育中扮演一定角色,并成功鉴定出7个与生长发育相关的关键基因。随着发育过程的推进,mTOR信号通路基因在不同发育时期的表达呈现出动态变化。其中,PI3K、TSC1/2和mTOR基因的整体表达变化趋势为先上升后下降再上升,IGFI变化趋势为先上升后下降,而EIF4B、RPS6KB和AKT基因表达则整体呈现下降趋势。这一差异化的基因表达模式反映了mTOR信号通路可能在厚壳贻贝幼虫不同发育时期中对细胞命运和生物学功能的调控,从而对其生长发育产生影响。因此,我们对mTOR信号通路关键基因在厚壳贻贝幼虫生长发育中的表达模式进行了初步探究。这些基因在调控厚壳贻贝幼虫的分子功能和生长特征方面具有重要作用,为深入理解海洋双壳动物的生理适应、代谢过程和生长变异提供了基础数据。In the field of aquaculture,a comprehensive exploration of the growth and developmental mechanisms of Mytilus coruscus lays the theoretical foundation for molecular regulation of growth and development.In order to investigate the expression patterns of genes associated with the growth and development differences in M.coruscus larvae,this study employed transcriptome sequencing and real-time quantitative PCR analysis techniques,conducting a preliminary examination of the differential expression of genes and molecular pathways related to growth characteristics.The research places particular emphasis on the impact and regulatory roles of the mammalian target of rapamycin(mTOR)signaling pathway in the growth and development of M.coruscus larvae.By analyzing the gene expression patterns at different developmental stages(Trochophore larvae stage,D-veliger larvae stage,Veliconcha larvae stage,Pediveliger larvae stage,and Juvenile stage),the results suggest that the mTOR signaling pathway may play a specific role in the growth and development of M.coruscus larvae.Seven key genes associated with growth were successfully identified.As the developmental process progresses,the expression of genes in the mTOR signaling pathway exhibits dynamic changes across different developmental stages.Among these changes,the expression levels of the PI3K,TSCI/2,and mTOR genes initially increased,followed by a decrease,and subsequently rose again.In contrast,IGFI expression exhibited an initial increase followed by a decline.Meanwhile,the expression of EIF4B,RPS6KB,and AKT genes demonstrated an overall downward trend.This differential gene expression pattern reflects the potential regulatory influence of the mTOR signaling pathway on cell fate and biological functions during various developmental stages of M.coruscus larvae,thereby impacting their growth and development.Therefore,this study provides a preliminary exploration of the expression patterns of key genes in the mTOR signaling pathway during the growth and development of M.coruscu

关 键 词：厚壳贻贝 MTOR信号通路 基因 生长发育 

分 类 号：S944.3[农业科学—水产养殖]