碳量子点(CDs)耦合Annexin-V抗体复合物活体SD大鼠周围神经功能束鉴别的基础研究  

Identification of Peripheral Nerve Functional Fascicles in Sprague-Dawley(SD)Rats by Carbon Quantum Dots(CDs)Coupled with an Annexin-V Antibody Complex

作　　者：孟宪宇[1] 李秋红[2] 刘岩[1] 张旭[1] 王磊[2] 姜巍 李凤久 MENG Xianyu;LI Qiuhong;LIU Yan;ZHANG Xu;WANG Lei;JIANG Wei;LI Fengjiu(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang University of Chinese Medicine,Harbin 150036,China)

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040 [2]黑龙江中医药大学,哈尔滨150036

出　　处：《神经损伤与功能重建》2024年第9期497-500,共4页Neural Injury and Functional Reconstruction

基　　金：黑龙江省自然科学基金联合引导项目(碳量子点(CDs)偶联Annexin-V抗体复合物活体Sprague-Daw-ley大鼠周围神经功能束鉴别的基础研究,No.LH2019H050);黑龙江省中医药科研项目(自研一体化夹板规范化治疗桡骨远端骨折的临床研究,No.ZHY19-032);黑龙江中医药大学教育教学研究基金项目(基于疾速原型制造技术的临床实践教学法在中医骨伤科课程中的实施与探索,No.XJJ2014006);高等学校博士学科点专项科研基金(应用Annexin V抗体偶联CdTe纳米量子点进行周围神经感觉与运动束鉴别的基础研究,No.20132327120004);哈尔滨市科学技术局科技计划项目(3D关节成形垫片微型外固定组合架治疗手部指间关节粉碎性骨折(Pilon骨折)的应用研究,No.2022ZCZJNS057)。

摘　　要：目的:探寻术中可用的周围神经功能束鉴别的方法。方法:分组制备SD大鼠双侧股神经主干、股神经肌支及隐神经Sunderland V损伤模型,制备的Annexin V-碳量子点(Carbon quantumdots,CDs)抗体复合物涂抹神经断端,在380 nm紫外灯激发光源照射下,5、10、15和20 min后在荧光显微镜下观察荧光显色。结果:SD大鼠双侧股神经主干、股神经肌支及隐神经Sunderland V损伤断端染色5 min荧光强度不足以充分显示神经束;染色10~15 min强度明显增加,股神经主干的感觉束及隐神经在Annexin V-CDs抗体复合物染色下呈蓝色荧光,股神经主干的运动束及股神经肌支无荧光显示;染色20 min后荧光强度逐渐减弱。各分组组内样本染色5、10、15、20 min的染色强度无明显差异。结论:Annexin V-CDs抗体复合物能够实现术中SD大鼠周围神经功能束的鉴别。Objective:To explore a method to rapidly differentiate between the sensory and motor fascicles in peripheral nerves for clinical application.Methods:The Sunderland V injury model was induced in the bilateral femoral nerve trunk,the muscle branch of the femoral nerve and the saphenous nerve of Sprague-Dawley(SD)rats.The Annexin V-Carbon quantum dots(CDs)antibody complex was prepared and applied to the severed nerve ends.Fluorescence was assessed under a 380 nm UV light source at 5,10,15 and 20 minutes after staining under a fluorescence microscope.Results:The fluorescence intensity at 5 minutes was insufficient to fully display nerve bundles in the injured bilateral femoral nerve trunk,the muscle branch of the femoral nerve,and the saphenous nerve of SD rats.However,the intensity of staining increased significantly between 10 to 15 minutes,with the sensory fascicles of the femoral nerve trunk and saphenous nerve showing blue fluorescence upon Annexin V-CDs antibody complex staining,while no fluorescence was observed in the motor fascicles of the femoral nerve trunk or the muscle branch of the femoral nerve.The fluorescence intensity gradually decreased after 20 minutes.There were no significant differences in the staining intensity within each group at 5,10,15 or 20 minutes.Conclusion:The Annexin V-CDs antibody complex can be used to effectively distinguish sensory from motor fascicles in peripheral nerves intraoperatively in SD rats.

关 键 词：周围神经 感觉束与运动束 鉴别 ANNEXIN-V 碳量子点 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.049[医药卫生—临床医学]