灵芝多糖饮料对高脂联合STZ诱导的糖尿病小鼠糖脂代谢的改善作用  

Improvement in the Glycolipid Metabolism of Diabetic Mice Induced by High-fat Diet with Streptozotocin Supplemented with Ganoderma lucidum Polysaccharide Beverage

作　　者：李佳雪 李程 宋晓兵 陈彦君 叶诗怡 张碧莹 陈萍[1] LI Jiaxue;LI Cheng;SONG Xiaobing;CHEN Yanjun;YE Shiyi;ZHANG Biying;CHEN Ping(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130000,China;Jilin Agricultural Science and Technology University,Changchun 130000,China;Jilin Zhongke Special Food Innovation Research Institute Corporation,Changchun 130000,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130000 [2]吉林农业科技学院,吉林长春130000 [3]吉林省中科特殊食品创新研究院有限公司,吉林长春130000

出　　处：《食品工业科技》2024年第19期9-16,共8页Science and Technology of Food Industry

基　　金：吉林省中科特殊食品创新研究院项目(2022220001000002)。

摘　　要：为探究长白山赤灵芝子实体多糖饮料对糖尿病小鼠的糖脂代谢的影响及保护作用。本研究利用高脂饮食结合腹腔注射链脲佐菌素诱导法建立糖尿病小鼠模型,设立正常对照组、模型对照组,并以盐酸二甲双胍为阳性对照,灌胃低、中、高不同剂量的灵芝多糖饮料探究对糖尿病小鼠的糖脂代谢改善作用,测定经灵芝多糖饮料作用后小鼠的空腹血糖值、胰岛素抵抗指数以及甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、超氧化物歧化酶活力、丙二醛等相关指标的变化情况。结果表明:与模型组相比,灵芝多糖饮料低、中、高剂量组的小鼠空腹血糖值分别下降了35.46%、39.05%、42.28%,胰岛素抵抗指数分别极显著(P<0.01)下降43.10%、50.25%、54.18%;高剂量组小鼠甘油三酯、低密度脂蛋白胆固醇含量分别下降46.83%、50.79%,高密度脂蛋白胆固醇上升107.81%;此外,灵芝多糖饮料能够提高糖尿病小鼠血清中超氧化物歧化酶活力,极显著降低丙二醛含量(P<0.01)其中L-GLB组、M-GLB组、H-GLB组分别下降18.66%、30.15%、44.54%,因此,灵芝多糖饮料可以缓解糖尿病引发的氧化应激,改善糖尿病引起的脏器肿大,保护DM小鼠的肝脏、肾脏和脾脏,在一定程度上能够改善由长期高血糖导致的肝脏损伤。This study aimed to investigate the effects of Changbaishan Ganoderma lucidum seed body polysaccharide beverage on the glucose and lipid metabolism of diabetic mice.High-fat diet combined with intraperitoneal injection of streptozotocin was used to establish a diabetic mouse model.A normal control group and a model control group were also set up.Metformin hydrochloride was used as a positive control.G.lucidum polysaccharide beverage was administered at different doses of low(L-GLB),medium(M-GLB),and high(H-GLB)to investigate its improvement effects on the glucose and lipid metabolism of diabetic mice.Fasting blood glucose,insulin resistance index,triglyceride,high-and low-density lipoprotein cholesterol,superoxide dismutase activity,and malondialdehyde were measured in the mice after receiving the G.lucidum polysaccharide beverage.Compared with those of the model group,the fasting blood glucose levels of the mice in the L-GLB,M-GLB,and H-GLB groups decreased by 35.46%,39.05%,and 42.28%,respectively,and their homeostatic model assessment of insulin resistance decreased by 43.10%,50.25%,and 54.18%,respectively(P<0.01).The triglyceride levels and LDL cholesterol of the mice in the high-dose group decreased by 46.83%and 50.79%,respectively,and their HDL cholesterol increased by 107.81%.G.lucidum polysaccharide beverage improved the superoxide dismutase activity in the serum of diabetic mice and significantly reduced the malondialdehyde content(P<0.01)by 18.66%,30.15%,and 44.54%in the L-GLB,M-GLB,and H-GLB groups,respectively.Therefore,G.lucidum polysaccharide beverage can relieve diabetes-induced oxidative stress and organ enlargement,protect the liver,kidney and spleen of diabetic mice,and improve the liver damage caused by long-term hyperglycemia to a certain extent.

关 键 词：灵芝多糖饮料 糖尿病模型 糖脂代谢 小鼠实验 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]