LncRNA FER1L4与胃癌细胞多药耐药的关系及机制探究  

Relationship Between LncRNA FER1L4 and Multi-drug Resistance of Gastric Cancer Cells and Its Mechanism

作　　者：王亚楠 WANG Yanan(Department of Oncology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453001,China)

机构地区：[1]新乡医学院第一附属医院肿瘤科,河南新乡453001

出　　处：《河南医学研究》2024年第18期3265-3268,共4页Henan Medical Research

摘　　要：目的探究长链非编码RNA(LncRNA)FER1L4与胃癌细胞多药耐药的关系及潜在机制。方法培养胃癌亲本细胞系SGC7901、SGC7901多柔比星抵抗细胞系(SGC7901/ADR)及SGC7901长春新碱抵抗细胞系(SGC7901/VCR),将SGC7901/ADR分为对照A组及实验A组,SGC7901/VCR分为对照B组及实验B组,SGC7901作为亲本组,通过慢病毒转染法将Plvx-Puro载体转染至亲本组、对照A组及对照B组中,Plvx-FER1L4-Puro载体转染至实验A组及实验B组中;MTT法检测细胞对ADR及VCR的敏感性,实时荧光定量PCR检测细胞中LncRNA FER1L4及三叶因子1(TFF1)mRNA表达,蛋白印迹实验检测TFF1及核因子κB(NF-κB)p65的蛋白表达。结果相比亲本组,对照A组及实验A组ADR半抑制浓度(IC50)增高(P<0.05),且实验A组低于对照A组(P<0.05);相比亲本组,对照B组及实验B组VCR IC50增高(P<0.05),且实验B组低于对照B组(P<0.05);相比亲本组,对照A、B组FER1L4及TFF1表达下调(P<0.05),实验A、B组FER1L4及TFF1表达增高(P<0.05),对照A、B组及实验A、B组p65蛋白表达均增高(P<0.05)。相比对照A、B组,实验A、B组FER1L4及TFF1表达均增高(P<0.05),而p65蛋白降低(P<0.05)。结论LncRNA FER1L4可促进抑癌基因TFF1的表达,抑制胃癌细胞多药耐药能力。Objective To explore the relationship between long non-coding RNA(lncRNA)FER1L4 and multi-drug resistance of gastric cancer cells and related potential mechanism.Methods SGC7901,SGC7901 doxorubicin resistant cell line(SGC7901/ADR)and SGC7901 vincristine resistant cell line(SGC7901/VCR)were cultured.SGC7901/ADR cells were divided into control A and test A group,and SGC7901/VCR cells were divided into control B and test B group.SGC7901 was used as parent group.Plvx-Puro vector was transfected into parent group,control group A and control group B by lentivirus transfection,while plvx-FER1L4-puro vector was transfected into test A and B group.MTT method was used to detect the sensitivity of each group cells to ADR and VCR.Real-time fluorescent quantitative PCR was used to detect the expressions of lncRNA FER1L4 and trefoil factor 1(TFF1)mRNA,and the protein expressions of TFF1 and NF-κB p65 were detected by Western blot.Results Compared with the parent group,the 50%inhibitory concentrations(IC50)of ADR in control A and test A group were higher(P<0.05),and that in test A was lower than that in control A group(P<0.05).Compared with the parent group,the IC50 of ADR in control B and test B group were higher(P<0.05),and that in test B was lower than that in control B group(P<0.05).Compared with the parent group,the expressions of FER1L4 and TFF1 in control A,B group were decreased(P<0.05),and in test A,B group were increased(P<0.05),while the expression of p65 protein was increased in control A,B and test A,B group(P<0.05).Compared with control A,B group,the expression of FER1L4 and TFF1 in test A,B group was increased(P<0.05),while the expression of p65 protein was decreased(P<0.05).Conclusion LncRNA FER1L4 can promote the expression of tumor suppressor gene TFF1 and inhibit the multi-drug resistance of gastric cancer cells.

关 键 词：胃癌 化疗抵抗 长链非编码RNA FER1L4 三叶因子1 

分 类 号：R735.2[医药卫生—肿瘤]