SV2B过表达对胶质母细胞瘤生物学行为的影响及其机制  

Effect of SV2B Overexpression on Biological Behavior of Glioblastoma and Its Mechanism

作　　者：陈倩 徐长玉 陈祺宁 段虎斌[1] CHEN Qian;XU Changyu;CHEN Qining;DUAN Hubin(Department of Neurosurgery,First Hospital of Shanxi Medical University,Taiyuan 030001,China;Department of Cardiothoracic Surgery,First Hospital of Qiqihar Medical University,Qiqihar 161000,China)

机构地区：[1]山西医科大学第一临床医学院神经外科,太原030001 [2]齐齐哈尔医学院附属第一医院心胸外科,齐齐哈尔161000

出　　处：《肿瘤防治研究》2024年第9期737-743,共7页Cancer Research on Prevention and Treatment

基　　金：山西省基础研究计划项目-面上项目(20210302124399);吕梁市重点研发计划(社会发展领域)项目(2020SHFZ37);山西省人社厅留学人员科技活动择优资助项目(晋人社厅函[2021]479,20210020)。

摘　　要：目的探讨SV2B过表达对胶质母细胞瘤细胞生长、侵袭及凋亡的影响及其潜在机制。方法通过慢病毒转染胶质母细胞瘤u87、u251细胞构建SV2B过表达组,另设空白对照组。CCK-8实验、细胞划痕实验、Transwell侵袭和迁移实验分别检测过表达SV2B对u87、u251细胞增殖、迁移和侵袭能力的影响,qRT-PCR和Westen blot检测SV2B蛋白表达水平。结果与空白对照组相比,过表达SV2B组胶质母细胞瘤细胞增殖、侵袭及迁移能力显著降低(P<0.05)。结论SV2B过表达显著抑制胶质母细胞瘤细胞增殖、侵袭和迁移能力。Objective To analyze the effect of SV2B overexpression on the growth,invasion,and apoptosis of glioblastoma cells,and to explore its potential mechanism.Methods We transfected glioblastoma u87 and u251 cells with lentivirus as SV2B overexpression group.And blank control group was set up.The effects of SV2B overexpression on the proliferation,migration,and invasion of u87 and u251 cells were detected by CCK-8 assay,cell scratch assay,Transwell invasion,and Transwell migration assay.The expression level of SV2B protein was detected by qRT-PCR and Western blot.Results Compared with the blank control group,the proliferation,invasion,and migration abilities of u87 and u251 cells in SV2B overexpression group were significantly reduced(P<0.05).Conclusion SV2B overexpression significantly inhibits the proliferation,invasion,and migration abilities of glioblastoma cells.

关 键 词：胶质母细胞瘤 SV2B 肿瘤预后 恶性进展 

分 类 号：R739.41[医药卫生—肿瘤]