载黄连素的同轴静电纺丝膜对牙周致病菌及生物膜的抑菌性研究  

Antibacterial property of berberine-loaded coaxial electrospun membranes against periodontal pathogens and biofilms

作　　者：张睿 郝婷 吕闻 任双双[2] 刘玉[3] 吴文蕾[3] 孙卫斌[1] Zhang Rui;Hao Ting;LüWen;Ren Shuangshuang;Liu Yu;Wu Wenlei;Sun Weibin(Dept.of Periodontics,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China;Dept.of Cariology and Endodontics,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China;Dept.of Senior Specialist,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院、南京市口腔医院牙周病科、南京大学口腔医学研究所,南京210008 [2]南京大学医学院附属口腔医院、南京市口腔医院牙体牙髓病科、南京大学口腔医学研究所,南京210008 [3]南京大学医学院附属口腔医院、南京市口腔医院高级专家诊疗科、南京大学口腔医学研究所,南京210008

出　　处：《国际口腔医学杂志》2024年第5期596-607,共12页International Journal of Stomatology

基　　金：国家自然科学基金面上项目(51972167)。

摘　　要：目的制备并表征负载黄连素的同轴静电纺丝膜,评价其体外抑制牙周致病菌和生物膜的作用。方法采用同轴静电纺丝法制备负载黄连素的聚己内酯/明胶膜(PCL/Gel-BBR)并对其进行表征检测,黄连素负载质量分数分别为0.25%、0.5%、1.0%。通过抑菌试验评价载药膜对牙龈卟啉单胞菌(P.gingivalis,ATCC 33277)及具核梭杆菌(F.nucleatum,ATCC 25586)增殖的抑制作用。采用结晶紫染色法、苯酚-硫酸法、菌落计数法、活/死细菌染色等综合评价载药膜对P.gingivalis和F.nucleatum双菌种生物膜的影响。采用CCK-8法和共聚焦显微镜检测载药膜的细胞毒性。结果同轴黄连素载药膜理化性能良好且能在体外稳定释放黄连素。1.0%黄连素载药膜可以有效抑制P.gingivalis和F.nucleatum的增殖,对双菌种生物膜的形成和活性也有抑制作用,且以上效应呈现浓度依赖性。黄连素载药膜体外生物相容性良好。结论负载黄连素的同轴静电纺丝膜具备体外抑制牙周致病菌进而抑制生物膜形成的作用,生物相容性良好,有望用于牙周药物递送以辅助牙周炎治疗或预防牙周术后感染。Objective This study aimed to prepare berberine-loaded coaxial electrospun membranes,evaluate their antibacterial effects against periodontal pathogens,and explore their inhibitory effect on biofilm formation in vitro.Methods Coaxial polycaprolactone/gelatin electrospun membranes loaded with berberine(drug loading capacity of 0.25%,0.5%and 1.0%,respectively)were fabricated and characterized.The bacteriostatic zone test was used to evaluate the antibacterial effect against Porphy romonas gingivalis(ATCC 33277)and Fusobacterium nucleatum(ATCC 25586).Crystal violet staining,phenol-sulfuric acid method,colony counting method,and live/dead cell staining were used to comprehensively evaluate the inhibitory effect of drug-loaded electrospun membranes on biofilm formation.The cell counting kit-8 and confocal microscopy methods was used to evaluate the biocompatibility of electrospun membranes.Results The berberine-loaded electrospun membranes had stable physicochemical properties,and they could release berberine in vitro.The membranes(drug loading capacity of 1.0%)exhibited efficient antibacterial activity against Porphyromonas gingivalis and Fusobacterium nucleatum,and inhibited the formation of biofilms in a concentration-dependent manner.The berberine-loaded membranes had good biocompatibility in vitro.Conclusion The berberine-loaded coaxial electrospun membranes were biocompatible,and they had an antibacterial potential against periodontal pathogens and biofilm formation in vitro.Therefore,it was promising to use berberine-loaded membranes as adjunctive therapy or to prevent postoperative infection.

关 键 词：黄连素 同轴静电纺丝 牙龈卟啉单胞菌 生物膜 

分 类 号：R781.4[医药卫生—口腔医学]