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作 者:Mingrui LIN Zhicheng YU Jinshui LIN Kunxiu CAI Zhaoyang HUANG Xuejiao HE
机构地区:[1]Fujian Institute of Tropical Crops,Zhangzhou 363001,China
出 处:《Plant Diseases and Pests》2024年第4期37-39,共3页植物病虫害研究(英文版)
基 金:Supported by Special Project of Public-interest Scientific Institutions of Fujian Province(2021R1011003).
摘 要:[Objectives]The paper was to study the tissue culture nursery technology of Curcuma alismatifolia‘Kimono Rose’.[Methods]By sterilizing and inoculating explants derived from disparate regions of C.alismatifolia,we identified the most optimal explants and optimized the culture conditions for cluster buds induction and proliferation.This was achieved by incorporating MS medium with varying concentrations of 6-BA and NAA,thereby establishing a foundation for the large-scale production of C.alismatifolia tissue culture seedlings.[Results]The optimal explant for C.alismatifolia was identified as a lateral bud.The most effective cluster buds induction medium was determined to be MS+6-BA 5 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 6.5 g/L.The optimal cluster buds proliferation medium was found to be MS+6-BA 3 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 6.5 g/L.[Conclusions]The findings of this study can provide a foundation for the enhancement of the industrialized breeding system of tissue culture propagation of C.alismatifolia.
关 键 词:Curcuma alismatifolia EXPLANTS Cluster buds induction PROLIFERATION
分 类 号:S436.8[农业科学—农业昆虫与害虫防治]
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