耐锰短芽孢杆菌MM2介导的生物矿化过程及其对土霉素去除作用的研究  

Manganese-tolerance in Brevibacillus brevis MM2:A study on its bio-mineralization processes and efficacy in oxytetracycline removal

作　　者：李植妍 王进[1,2] 邓锐 陈明珠 刘阿钻 贺笑 岳正波 LI Zhiyan;WANG Jin;DENG Rui;CHEN Mingzhu;LIU Azuan;HE Xiao;YUE Zhengbo(School of Resources and Environmental Engineering,Hefei University of Technology,Hefei 230009;Anhui Engineering Research Center of Industrial Wastewater Treatment and Resource Recovery,Hefei University of Technology,Hefei 230009;Nanshan Mining Company Ltd,Anhui Maanshan Iron and Steel Mining Resources Group,Maanshan 243000)

机构地区：[1]合肥工业大学资源与环境工程学院,合肥230009 [2]安徽省工业废水处理与资源化工程研究中心,合肥230009 [3]安徽马钢矿业资源集团南山矿业有限公司,马鞍山243000

出　　处：《环境科学学报》2024年第9期206-218,共13页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.52070062,U20A20325)。

摘　　要：通过对酸性矿山废水中分离并纯化得到的耐锰短芽孢杆菌BrevibacillusbrevisMM2进行深入探究,旨在阐明其生物氧化锰形成机理及其对土霉素(OTC)的去除效能.首先运用全基因组测序结合KEGG功能注释分析揭示了菌株MM2在碳水化合物和氨基酸代谢途径中富含的功能基因簇,表明其具有高效的异养代谢潜能及卓越的环境适应能力,并可能参与关键的锰元素氧化调控机制.实验结果显示,在锰(Mn(Ⅱ))胁迫条件下,MM2菌株启动特异性应激反应,显著促进生物锰氧化物的生成及高效地吸附与转化锰离子.通过形态学表征与成分解析,证实了由菌株MM2所产生的生物氧化锰主要以无定形态结构存在,表现为附着于菌体表面的片状与颗粒状复合物,包含Mn_(3)O_(4)与Mn_(2)O_(3)等重要成分.批量实验结果表明,在pH为9时观察到生物氧化锰对OTC去除效果达到峰值.进一步的实验数据揭示了OTC去除速率与其投加量以及一定pH范围内的初始浓度呈正比例关系,但当OTC初始浓度增大时,其去除效率表现出负相关趋势.此外,OTC去除过程遵循一级反应动力学模型,去除作用中包括吸附和降解,微生物活性在此过程中起着关键的促进作用.这一理论发现为利用短芽孢杆菌MM2及其产生的生物氧化锰处理含OTC废水提供了坚实的科学依据和优化处理策略.In this study,we investigated Mn-resistant Brevibacillus brevis MM2 isolated and purified from acid mine wastewater with the aim of elucidating the mechanism of Mn-oxidation and its efficacy in removing oxytetracycline(OTC).Firstly,whole genome sequencing combined with KEGG functional annotation analysis revealed that strain MM2 is enriched in functional gene clusters in carbohydrate and amino acid metabolic pathways,indicating that it has efficient heterotrophic metabolic potential and excellent environmental adaptability,and may be involved in the key Mn oxidative regulatory mechanisms.The experimental results showed that under manganese(Mn(II))stress,MM2 strain initiated a specific stress response,which significantly promoted the production of Bio-MnOx and efficiently adsorbed and transformed manganese ions.Morphological characterization and compositional analysis confirmed that the Bio-MnOx produced by strain MM2 mainly existed in an amorphous structure,which was manifested as lamellar and granular complexes attached to the surface of the bacterium,and contained important components such as Mn_(3)O_(4)and Mn_(2)O_(3).The results of the batch experiments showed that the peak effect of Bio-MnOx oxide on OTC removal was observed at pH 9.Further experimental data revealed that the OTC degradation rate was positively proportional to its dosage and initial concentration in a certain pH range,but the removal efficiency showed a negative correlation trend when the initial concentration of OTC increased.In addition,the OTC removal process follows a first-order reaction kinetics model,and the removal process includes adsorption and degradation,in which microbial activity plays a key promoting role.This theoretical finding provides a solid scientific basis and an optimized treatment strategy for the treatment of OTC-containing wastewater using Brevibacillus brevis MM2 and its produced Bio-MnOx.

关 键 词：锰氧化细菌 生物氧化锰 土霉素 KEGG 

分 类 号：X172[环境科学与工程—环境科学]