基于工程化盐单胞菌TDZI-08一锅法合成衣康酸  

One-pot synthesis of itaconic acid by engineered Halomonas bluephagenesis TDZI-08

作　　者：张静[1,2] 元跃 王智文 陈涛[1] ZHANG Jing;YUAN Yue;WANG Zhiwen;CHEN Tao(Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering,Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;College of Agricultural and Biological Engineering,Heze University,Heze 274000,Shandong,China)

机构地区：[1]天津大学化工学院、教育部合成生物学前沿科学中心与系统生物工程重点实验室,天津300072 [2]菏泽学院农业与生物工程学院,山东菏泽274000

出　　处：《生物工程学报》2024年第8期2666-2677,共12页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2018YFA0900200)。

摘　　要：衣康酸(itaconic acid,IA)是12种高附加值平台化合物之一,广泛应用于涂料、黏合剂、塑料、树脂和生物燃料等领域。本研究基于前期构建的工程化盐单胞菌TDZI-08,通过排查阻碍因素、优化碳源、氮源、诱导剂添加时间和表面活性剂添加量等,建立了一锅法催化柠檬酸合成衣康酸工艺。在5 L发酵罐中利用TDZI-08进行开放式不灭菌一锅法合成,最高产生了40.50 g/L衣康酸,催化阶段得率为0.68 g衣康酸/g柠檬酸,总得率为0.42 g衣康酸/g(柠檬酸+葡萄糖酸)。本研究探索出的一锅法合成体系工艺简单且无须灭菌和无菌操作,表明盐单胞菌具有较好的衣康酸工业化生产潜力。Itaconic acid(IA)is one of the twelve high value-added platform compounds applied in various fields including coatings,adhesives,plastics,resins,and biofuels.In this study,we established a one-pot catalytic synthesis system for IA from citric acid based on the engineered salt-tolerant bacterial strain Halomonas bluephagenesis TDZI-08 after investigating factors that hindered the process and optimizing the carbon source,nitrogen source,inducer addition time,and surfactant dosage.The open,non-sterile,one-pot synthesis with TDZI-08 in a 5 L fermenter achieved the highest IA titer of 40.50 g/L,with a catalytic yield of 0.68 g IA/g citric acid during the catalytic stage and a total yield of 0.42 g IA/g(citric acid+gluconic acid).The one-pot synthesis system established in this study is simple and does not need sterilization or aseptic operations.The findings indicate the potential of H.bluephagenesis for industrial production of IA.

关 键 词：盐单胞菌 衣康酸 一锅法合成 工艺优化 

分 类 号：TQ921[轻工技术与工程—发酵工程]