丙酸的合成生物制造  

Biomanufacturing of propionic acid

作　　者：郑棚 闫更轩 王丽敏[1] 张云志[2] 陶勇[1] 于波[1] ZHENG Peng;YAN Gengxuan;WANG Limin;ZHANG Yunzhi;TAO Yong;YU Bo(Key Laboratory of Microbial Physiological&Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,Heilongjiang,China)

机构地区：[1]中国科学院微生物研究所微生物生理与代谢工程研究室,北京100101 [2]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010

出　　处：《生物工程学报》2024年第8期2678-2694,共17页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2018YFA0901400);国家自然科学基金(32070026);黑龙江省省属科研院所科研业务费项目(CZKYF2023SW04)。

摘　　要：丙酸是一种重要的C3平台化合物,在食品、药品和化工等领域应用广泛。以石油等化工产品为原料通过化学途径合成,环境污染严重且不可持续。近些年,利用微生物转化可再生资源生产丙酸受到了广泛关注。本文聚焦丙酸生物制造技术,首先综述了传统丙酸杆菌代谢工程改造和在大肠杆菌及酿酒酵母等异源宿主中重构丙酸合成途径的研究;其次重点讨论了基于合成生物学技术,通过对恶臭假单胞菌KT2440的途径设计和改造,实现其利用L-苏氨酸或生物基1,2-丙二醇为原料的高效生物催化合成高纯度丙酸的最新进展。Propionic acid as an important C3 platform chemical has been widely used in food,pharmaceutical,and chemical fields.The chemical synthesis of propionic acid from petroleum and other chemical products has serious environmental pollution and is not sustainable.In recent years,the production of propionic acid by microbial transformation of renewable resources has received extensive attention.Focusing on the biomanufacturing of propionic acid,this paper firstly reviews the studies about the metabolic engineering of Propionibacterium and the pathway reconstruction in heterogeneous hosts such as Escherichia coli and Saccharomyces cerevisiae.Secondly,this paper reviews the recent progress in the synthesis of high-purity propionic acid from L-threonine or bio-based 1,2-propanediol by the design and modification of the pathway of Pseudomonas putida KT2440 based on synthetic biology.

关 键 词：丙酸 代谢工程 L-苏氨酸 生物制造 

分 类 号：TQ921[轻工技术与工程—发酵工程]