天然甲醇化学品细胞工厂改造进展与展望  

Progress and perspectives of natural cell factories for chemical production from methanol

作　　者：汪淑贤 方嘉煜 张延平[1] 李寅[1] 朱泰承[1] WANG Shuxian;FANG Jiayu;ZHANG Yanping;LI Yin;ZHU Taicheng(State Key Laboratory of Microbial Resources,Department of Microbial Physiological&Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院微生物研究所微生物生理与代谢工程研究室微生物资源前期开发国家重点实验室,北京100101 [2]中国科学院大学,北京100049

出　　处：《生物工程学报》2024年第8期2747-2760,共14页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2021YFC2103500,2018YFA0901400);国家自然科学基金(32270058,31970039)。

摘　　要：甲醇因具有生产工艺成熟、价格低廉和可再生等优点,被认为是下一代生物制造的核心原料之一。通过构建高效的微生物细胞工厂将甲醇转化为化学品已经成为绿色生物制造行业的研究热点。本文聚焦天然甲醇细胞工厂,对非天然甲醇细胞工厂和天然甲醇细胞工厂进行了比较,讨论了天然甲醇细胞工厂存在的关键问题和挑战,综述了近年来围绕这些问题对天然甲醇细胞工厂进行改造的研究进展,并进一步展望了可能的解决方案,为未来天然甲醇细胞工厂的改造提供了可行的研究策略。Methanol has been considered one of the most important alternative carbon sources for the next-generation biomanufacturing due to its low price,mature production processes,and potential sustainability.Constructing microbial cell factories for methanol to chemical biotransformation has become a research hotspot in the green biomanufacturing industry.Focusing on the microorganisms that can naturally use methanol,we compare them with non-natural cell factories for chemical production from methanol.We discuss the key issues and challenges associated with natural cell factories for chemical production from methanol,summarize recent research progress surrounding these issues,and propose possible solutions to these challenges.This review helps to generate feasible guidelines and research strategies for the modification of natural cell factories for efficient methanol to chemical production in the future.

关 键 词：甲醇 生物转化 天然甲醇细胞工厂 合成生物学 化学品生产 

分 类 号：TQ223.121[化学工程—有机化工]