川芎活性物质阿魏酸乙酯联合有氧运动改善阿尔茨海默病大鼠的认知功能研究  

Effects of combination of Ligusticum chuanxiong active substance ethyl ferulate and aerobic exercise on cognitive function of Alzheimer’s disease rats

作　　者：董晋[1] 韩泽宇 DONG Jin;HAN Zeyu(School of Physical Education,Shanxi University,Shanxi Taiyuan 030006,China)

机构地区：[1]山西大学体育学院,山西太原030006

出　　处：《中国食品卫生杂志》2024年第6期659-666,共8页Chinese Journal of Food Hygiene

基　　金：2022年度山西省高等学校重点研究基地项目(2022J008)。

摘　　要：目的探讨川芎活性物质阿魏酸乙酯(EFE)联合有氧运动(AE)对阿尔茨海默病(AD)大鼠认知功能的影响及作用机制。方法将70只SD大鼠随机分为5组(n=12):对照组、模型组、AE组、EFE组和AE+EFE组。通过对大鼠双侧海马注射β-淀粉样蛋白1-42(Aβ1-42)诱导AD大鼠模型。建模1周后,AE组和AE+EFE组大鼠进行有氧跑台运动,其他组大鼠不运动;EFE组和AE+EFE组大鼠灌胃2 mL 100 mg/kg的阿魏酸乙酯,其他组大鼠灌胃2 mL 0.5%羧甲基纤维素钠,共灌胃6周。给药结束后,通过Morris水迷宫实验评估大鼠的认知功能。根据试剂盒说明检测海马组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)水平。利用qRT-PCR检测海马组织β-淀粉样前体蛋白裂解酶1(BACE1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、核因子红细胞2相关因子2(Nrf2)、Kelch样环氧氯丙烷相关蛋白-1(Keap1)和血红素加氧酶1(HO-1)转录水平。通过Western blot检测海马组织BACE1和Aβ1-42蛋白表达水平。结果与AE组和EFE组比较,AE+EFE组的逃避潜伏期降低,60 s内穿越平台的次数显著升高(P<0.05),海马组织SOD和CAT水平显著升高,海马组织MDA水平显著降低(P<0.05)。与AE组和EFE组比较,AE+EFE组的海马组织TNF-α、IL-1β、IL-6和Keap1mRNA相对表达量显著降低(P<0.05),而Nrf2和HO-1 mRNA相对表达量显著升高。与AE组和EFE组比较,AE+EFE组的海马组织BACE1 mRNA、BACE1蛋白和Aβ1-42蛋白相对表达量降低(P<0.05)。结论阿魏酸乙酯联合有氧运动可有效改善AD大鼠的认知功能,其机制可能与抑制氧化应激、炎症和Aβ的积累有关。Objective To investigate the effect of combination of Ligusticum chuanxiong active substance ethyl ferulate(EFE)and aerobic exercise(AE)on cognitive function of rats with Alzheimer’s disease(AD).Methods Seventy SD rats were randomly divided into 5 groups(Control group,Model group,AE group,EFE group and AE+EFE group n=12).The rat model of AD was induced by injectingβ-amyloid 1-42(Aβ1-42)into the hippocampus of rats on both sides.One week after modeling,the rats in AE group and AE+EFE group took aerobic treadmill exercise,while the other groups did not exercise.And the rats in EFE group and AE+EFE group were gavaged with 2 mL 100 mg/kg ethyl ferulate,the rats in other groups were gavaged with 2 mL 0.5%sodium carboxymethyl cellulose.All groups were treated for 6 weeks.After administration,the rats’cognitive function was assessed by the Morris Water maze test.The levels of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)in hippocampal tissue were detected according to the instructions of the kit.Hippocampalβ-amyloid precursor protein lyase 1(BACE1),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),nuclear factor erythrocyte 2-associated factor 2(Nrf2),Kelch-like ECHassociated protein 1(Keap1)and heme oxygenase 1(HO-1)transcription levels were detected by qRT-PCR.The expression levels of BACE1 and Aβ1-42 in hippocampus were detected by western blot.Results Compared with AE group and EFE group,the escape latency of AE+EFE group decreased,and the number of crossing the platform within 60 s increased significantly(P<0.05).Compared with AE group and EFE group,SOD and CAT levels in the hippocampus of AE+EFE group increased,while MDA levels in the hippocampus decreased significantly(P<0.05).Compared with AE and EFE groups,the mRNA relative expressions of TNF-α,IL-1βand IL-6 in hippocampus of AE+EFE group decreased significantly(P<0.05).Compared with AE and EFE groups,the relative expression levels of Nrf2 and HO-1 mRNA in the hippocampus of AE+EFE group were increased,whil

关 键 词：阿魏酸乙酯 有氧运动 阿尔茨海默病 认知功能 淀粉样Β蛋白 

分 类 号：R155[医药卫生—营养与食品卫生学]