基于基因组学的小海绵羊肚菌环境适应研究  

Research on the Environmental Adaptability of Morchella Spongiola Based on Genomics

作　　者：李艳艳 徐鸿雁[2] 谢占玲[1] 孟清 LI Yanyan;XU Hongyan;XIE Zhanling;MENG Qing(Qinghai University,Xining Qinghai 810016,China;Academy of Agriculture and Forestry Sciences,Qinghai University,Xining Qinghai 810016,China)

机构地区：[1]青海大学,青海西宁810016 [2]青海大学农林科学院,青海西宁810016

出　　处：《青海农林科技》2024年第3期8-16,共9页Science and Technology of Qinghai Agriculture and Forestry

基　　金：青海省科学技术厅项目(2021-HZ-802)。

摘　　要：本研究基于小海绵羊肚菌M12-10基因组数据库挖掘环境适应基因,探索其在高原极端环境中的适应机制。结果显示,通过KEGG注释数据库筛选出83条环境适应基因,主要涉及MAPK信号通路(60条)、磷脂酰肌醇信号系统(16条)和ABC转运蛋白类(7条)。通过BLAST和文献综述将这些基因分为低温、紫外、盐等8类环境因素相关因子,其中抗逆境胁迫基因最多,为44条,抗低氧胁迫基因最少,为4条。筛选的盐基因M12-10_G01390在1 mol/L低盐浓度胁迫下表达量最低,3 mol/L高盐浓度胁迫下时表达量相对较高(0.25);低温基因M12-10_G03567在4℃胁迫下表达量最高(1.9);紫外基因M12-10_G04909在短时间(30 min)紫外照射下表达量升高(1.34),在中长(60 min、120 min)、长时间(300 min)紫外照射下,其表达量下降(均在0.25以下)。本文为后续的羊肚菌在青藏高原生态环境中适应性研究提供参考。In this study,the environmental adaptation genes were mined based on the genome database of M12-10,and the adaptation mechanism in the extreme environment of the plateau was explored.The results showed that 83 environmental adaptation genes were screened through the KEGG annotation database,mainly involving MAPK signaling pathway(60),phosphatidylinositol signaling system(16)and ABC transporters(7).Through BLAST and literature review,these genes were divided into 8 types of environmental factor-related factors such as low temperature,ultraviolet,and salt.Among them,the most stress-resistant genes were 44,and the least anti-hypoxia stress genes were 4.The expression level of the screened salt gene M12-10_G01390 was the lowest under 1 mol/L low salt stress,and the expression level was relatively high under 3 mol/L high salt stress(0.25).The expression level of low temperature gene M12-10_G03567 was the highest(1.9)under 4℃temperature stress.The expression of UV gene M12-10_G04909 increased(1.34)under short time(30 min)UV irradiation,and decreased(all below 0.25)under medium(60 min,120 min)and long time(300 min)UV irradiation.This article provides a reference for future research on the adaptability of M.esculenta in the ecological environment of the Qinghai Tibet Plateau.

关 键 词：小海绵羊肚菌 环境适应相关基因 胁迫 基因组分析 

分 类 号：S646.7[农业科学—蔬菜学]