不同盐度胁迫下大黄鱼肝脏转录组比较分析  

Comparative transcriptome analysis of the liver in Larimichthys crocea under different salinity stresses

作　　者：王永红 曾霖 吉群 谢正丽 黄伟卿 宋炜[2] WANG Yonghong;ZENG Lin;JI Qun;XIE Zhengli;HUANG Weiqing;SONG Wei(School of Food and Biological Engineering,Bengbu University,Bengbu Anhui 233030,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China;School of Life Sciences,Ningde Normal University,Ningde Fujian 352100,China)

机构地区：[1]蚌埠学院食品与生物工程学院,安徽蚌埠233030 [2]中国水产科学研究院东海水产研究所,上海200090 [3]中国水产科学研究院渔业机械仪器研究所,上海200090 [4]宁德师范学院生命科学学院,福建宁德352100

出　　处：《海洋渔业》2024年第4期435-445,共11页Marine Fisheries

基　　金：青岛海洋科技中心山东省专项经费(2022QNLM30001);国家重点研发计划(2022YFD2401102,2019YFDO900904);中国水产科学研究院基本科研业务费(2020TD76)。

摘　　要：为探讨盐度变化对大黄鱼(Larimichthys crocea)肝脏基因转录表达的影响,将体质量为(59.68±1.32)g的大黄鱼分别在盐度25(对照组)、盐度36(高盐组)、盐度10(低盐组)的水体中暴露24 h,采用Illumina Hiseq 2500对肝脏样本进行转录组测序。结果显示,从高盐vs.对照组(H vs.C)、低盐vs.对照组(L vs.C)和高盐vs.低盐(H vs.L)中分别筛选出71个、180个和438个差异基因(DEGs)。GO(Gene Ontology)和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析发现,H vs.C中的DEGs显著富集在三羧酸循环、MAPK信号通路、内质网蛋白加工和Toll样受体信号通路等;L vs.C中的DEGs显著富集在FoxO信号通路、戊糖磷酸途径、mTOR信号通路、RNA降解、P53信号通路和内质网蛋白加工等,表明大黄鱼对高盐和低盐胁迫的响应均涉及到糖类代谢、内质网应激、细胞凋亡和自噬等生理功能。H vs.L中的DEGs显著富集在内质网蛋白加工、FoxO信号通路、甘油脂代谢、糖酵解/糖异生、吞噬体和P53信号通路等,表明大黄鱼在高盐和低盐适应过程中,内质网应激、脂类代谢、糖类代谢、细胞凋亡和自噬等生理功能存在差异。研究结果可为深入研究鱼类对盐度胁迫的响应机制奠定基础。Large yellow croaker Larimichthys crocea is an important marine fish species,the annual yield of which is the highest among marine cage-farmed fish species in China.To improve the economic benefits of aquaculture,high stocking density of this species is conducted in practice,which often leads to white spot disease outbreaks.The reduction of salinity can promote fish growth and reduce the risk of Cryptocaryon irritans infection.However,little information is available on the molecular mechanism of positive effects of reducing salinity on fish.The purpose is to investigate the effects of salinity on gene transcriptome in the liver of L.crocea.Fish with an average weight of(59.68±1.32)g were transferred from salinity 25 to salinity 36 or salinity 10.Three groups were set:control group(C group),high-salinity group(H group)and low-salinity group(L group),each group with three replicates.After exposure for 24 h,liver was sampled for the transcriptome sequencing.The results showed that 71,180 and 438 differentially expressed genes(DEGs)were obtained in high-salinity group vs.control group(H vs.C),low-salinity group vs.control group(L vs.C),and high-salinity group vs.low-salinity group(H vs.L),respectively.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that DEGs in H vs.C were significantly enriched in citrate cycle,protein processing in endoplasmic reticulum,mitogen-activated protein kinase(MAPK)signaling pathway and Toll-like receptor signaling pathway.Citrate cycle is an important aerobic metabolic pathway,which can efficiently provide energy.Protein processing in endoplasmic reticulum can repair damaged cells and maintain correct protein folding.MAPK and Toll-like receptor signaling pathway can stimulate non-specific immune responses by activating multiple immune cells.Thus,fish adapted to high-salinity stress by regulating energy metabolism,non-specific immunity and endoplasmic reticulum stress.DEGs in L vs.C were significantly enriched in forkhead box O(FoxO)signaling path

关 键 词：大黄鱼 肝脏 盐度胁迫 转录组 差异基因 

分 类 号：S917.4[农业科学—水产科学]