三重四级杆液质联用测定乳酸环丙沙星中哌嗪的残留量  

Determination of Piperazine in Ciprofloxacin Lactate by Triple Quadrupole Liquid Mass Spectrometry

作　　者：杨帅 李欣 于亮 李霞 杨海霞 YANG Shuai;LI Xin;YU Liang;LI Xia;YANG Hai-xia(Shandong Academy of Pharmaceutical Sciences,Jinan 250101,China;NMPA Key Laboratory for Technology Research and Evaluation of Drug Products,Jinan 250101,China)

机构地区：[1]山东省药学科学院,山东济南250101 [2]国家药监局药物制剂技术研究与评价重点实验室,山东济南250101

出　　处：《精细化工中间体》2024年第4期82-84,共3页Fine Chemical Intermediates

摘　　要：建立液质联用法测定乳酸环丙沙星中哌嗪残留量的方法。采用C18色谱柱分离,串联四级杆质谱多反应离子监测方式检测,外标法定量。哌嗪浓度在0.021~1.28μg/m L范围内与峰面积线性关系良好(r=0.998),平均回收率为98.9%(RSD=1.61%)。方法专属性强、重复性好、灵敏度高,可用于乳酸环丙沙星中哌嗪残留量测定。To establish a method for the determination of piperazine in ciprofloxacin lactate by liquid mass spectrometry,samples were separated by C18 chromatographic column,detected by tandem quadrupole mass spectrometry multi-reactive ion monitoring,and quantified by external standard method.A good linear relationship was obtained in the concentration range of 0.021~1.28μg/mL for piperazine,with the correlation efficiency of 0.998.The average recovery was 98.9%(n=6),with an RSD of 1.61%.This method has good specificity,repeatability and high sensitivity.It can be used for the determination of residual piperazine in ciprofloxacin lactate.

关 键 词：液质联用 哌嗪 乳酸环丙沙星 

分 类 号：TQ460.7[化学工程—制药化工] R914[医药卫生—药物化学]