异荭草素-玉米醇溶蛋白/阿拉伯胶纳米纤维膜的制备表征及其愈伤作用  

The effect of isoorientin-zein/Arabic gum composite nanofiber membrane on wound healing in mice

作　　者：何紫妍 张萌 贾颖宇 杨胜薇 骆莹 王晓宇[1] 袁莉[1] HE Ziyan;ZHANG Meng;JIA Yingyu;YANG Shengwei;LUO Ying;WANG Xiaoyu;YUAN Li(School of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,Shaanxi,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2024年第5期71-81,共11页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金(31972183);陕西省技术创新引导专项(2022QFY09-03);西安市科技计划项目(21XJZZ0008)。

摘　　要：为探究异荭草素(isoorientin,ISO)与玉米醇溶蛋白(zein)/阿拉伯胶(Arabic gum,GA)制得的复合纳米纤维膜对小鼠皮肤损伤愈合的影响,以空白纺丝膜为对照,分别使用负载zein/GA的纳米纤维膜、负载ISO的纳米纤维膜以及负载ISO-zein/GA的复合纳米纤维膜对小鼠伤口进行敷料处理。结果表明,负载zein/GA的纳米纤维膜、负载ISO的纳米纤维膜以及负载ISO-zein/GA的复合纳米纤维膜对小鼠的伤口愈合具有促进作用,可以有效减少伤口氧化损伤及促炎因子TNF-α、IL-1β、iNOS的mRNA表达水平,并促进伤口血管重建,从而加速伤口愈合。研究结果验证了异荭草素-玉米醇溶蛋白/阿拉伯胶复合纳米纤维膜对伤口愈合的促进作用,拓宽了异荭草素的应用范围,为新型伤口敷料的研究提供了思路。To investigate the effect of composite nanofiber membrane prepared from isoorientin(ISO)and zein/Arabic gum(GA)on skin injury healing in mice,this study used blank spinning membrane as control,and wound dressings were applied to mice using zein/GA loaded nanofiber membrane,ISO loaded nanofiber membrane,and ISO-zein/GA loaded composite nanofiber membrane,respectively.The results showed that the nanofiber membranes loaded with zein/GA,ISO and ISO-zein/GA had promoting effects on wound healing in mice.They effectively reduced wound oxidative damage and mRNA expression levels of pro-inflammatory factors(TNF-α、IL-1β、iNOS),and promoted wound vascular reconstruction,thereby accelerating wound healing.The research results verify the promoting effect of ISO-zein/GA composite nanofiber membrane on wound healing,expand the application range of isoorientin,and provide ideas for the research of new wound dressing.

关 键 词：异荭草素 伤口愈合 静电纺丝 玉米醇溶蛋白 纳米纤维膜 

分 类 号：TS207.3[轻工技术与工程—食品科学]