An aptamer-driven DNA nanodevice for improved delivery of synthetic immunostimulants  

作　　者：Dejie Lu Zhenghan Di Lele Li Jian Zhao Li Zheng 

机构地区：[1]Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration,Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application,Guangxi Key Laboratory of Regenerative Medicine,The First Affiliated Hospital of Guangxi Medical University,Guangxi Medical University,Nanning 530021,China [2]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,National Center for Nanoscience and Technology,Beijing 100190,China

出　　处：《Nano Research》2024年第10期9078-9083,共6页纳米研究（英文版）

基　　金：supported financially by the National Natural Science Foundation of China(Nos.T2322005 and 32101156);Youth Innovation Promotion Association CAS.

摘　　要：Efficient delivery of therapeutics to immune cells remains a formidable challenge for cancer immunotherapy.In this work,we demonstrate that an aptamer-driven DNA nanodevice,constructed through linkage of a synthetic immunostimulant(Toll-like receptor 9 agonist:CpG motif)to an aptamer,could significantly enhance the immunostimulatory activity by facilitating the uptake and retention of therapeutics in macrophages.Systemic administration of the DNA nanodevice results in efficient tumor growth inhibition in both breast cancer and melanoma mouse models.Our studies suggest that the DNA nanodevice leads to reeducation of tumor-associated macrophages and ultimately to reversing the tumor immune microenvironment.The strategy for aptamer-mediated and vehicle-free delivery of immunostimulatory oligonucleotides provides a potential platform for cancer immunotherapy.

关 键 词：APTAMER immunostimulatory oligonucleotides DNA nanodevice tumor-associated macrophages cancer therapy 

分 类 号：TB383[一般工业技术—材料科学与工程]