人源性胰母细胞瘤异种移植模型的构建与验证  

Construction and validation of a patient-derived pancreatoblastoma xenograft model

作　　者：刘宁 孟肖振[1] 阎金龙 项灯 李匡凡 罗鸣 张守华[4] LIU Ning;MENG Xiao-zhen;YAN Jin-long;XIANG Deng;LI Kuang-fan;LUO Ming;ZHANG Shou-hua(Graduate School of Nanchang University,Nanchang 330038,Jiangxi,CHINA;Department of Neonatology,Jiujiang Maternal and Child Health Hospital,Jiujiang 332000,Jiangxi,CHINA;Department of General Surgery,the Second Affiliated Hospital of Nanchang University,Nanchang 330038,Jiangxi,CHINA;Department of General Surgery,Jiangxi Provincial Children's Hospital,Nanchang 330038,Jiangxi,CHINA)

机构地区：[1]南昌大学研究生院,江西南昌330038 [2]九江市妇幼保健院新生儿科,江西九江332000 [3]南昌大学第二附属医院普外科,江西南昌330038 [4]江西省儿童医院普外科,江西南昌330038

出　　处：《海南医学》2024年第18期2585-2589,共5页Hainan Medical Journal

基　　金：江西省卫生健康委员会科技计划项目(编号:202210069、202130879);江西省应用研究培育计划(编号:20212BAG70003)。

摘　　要：目的研究构建合适的动物模型用于胰母细胞瘤(PBL)的化疗药物筛选及新药评估。方法将手术切除的PBL肿瘤组织碎块移植到免疫缺陷的小鼠皮下组织,待移植瘤生长至1500 mm^(3)时进行瘤体传代及保存。使用HE染色、免疫组织化学及PCR法对成功建立的PBL患儿起源的异种移植(PDX)模型进行人源性鉴定。结果HE染色显示小鼠PDX中的肿瘤组织及细胞和患儿肿瘤细胞形态基本相同,免疫组化结果显示β-连环蛋白(β-Catenin)、细胞角蛋白7(CK7)和细胞角蛋白19(CK19)在PDX模型组织与人属源性肿瘤组织中有相同的免疫学特征。PCR分析显示PDX组织和原发肿瘤组织具有相同的个体来源。结论所建立胰母细胞瘤来源PDX高度保留了原发胰母细胞瘤的生长模式及蛋白表达特性,为胰母细胞瘤患者的基础研究和临床用药指导提供了重要的实验模型工具,给推动疾病机制探索及制定个性化治疗方案提供了科学依据。Objective To establish a suitable animal model for screening chemotherapeutic drugs and evaluating new drugs for pancreatoblastoma(PBL).Methods The fragments of PBL tumor tissues were transplanted into the subcutaneous tissue of immunodeficient mice.When the transplanted tumor grew to 1500 mm^(3),the tumor was passaged and preserved.The xenograft(PDX)model derived from PBL was successfully constructed.HE staining,immunohistochemistry,and PCR were used to identify the humanized PDX model.Results HE staining showed that the tumor tissues and cells in PDX mice were basically the same as those in children.Immunohistochemistry showed that βCatenin,CK7,and CK19 had the same immunological characteristics in PDX model tissues and human derived tumor tissues.PCR analysis revealed that PDX tissue and primary tumor tissue had the same individual origin.Conclusion The established pancreatoblastoma PDX highly retains the growth pattern and protein expression characteristics of primary pancreatoblastoma,which provides an important experimental model tool for basic research and clinical medication guidance for patients with pancreatoblastoma,and provides a scientific basis for promoting the exploration of disease mechanisms and formulating personalized treatment plans.

关 键 词：胰母细胞瘤 人源性肿瘤异种移植 模型构建 组织病理学 药物筛选 

分 类 号：R735.9[医药卫生—肿瘤]