小凹蛋白-1、小凹蛋白-2在宫颈鳞状细胞癌及鳞状上皮内病变中的表达及意义  

Expression and Significance of Caveolin-1 and Caveolin-2 in Cervical Squamous Cell Carcinoma and Squamous Intraepithelial Lesions

作　　者：赵慧 罗小林 ZHAO Hui;LUO Xiaolin(Department of Pathology,Urumqi Maternal and Child Health Hospital,Urumqi 830001,China;Department of Pathology,Karamay Hospital of Integrated Traditional Chinese and Western Medicine,Karamay 834000,China)

机构地区：[1]乌鲁木齐市妇幼保健院病理科,乌鲁木齐830001 [2]克拉玛依市中西医结合医院病理科,新疆克拉玛依834000

出　　处：《医学综述》2024年第20期2551-2555,2560,共6页Medical Recapitulate

基　　金：乌鲁木齐市卫生健康委科技计划项目(202107)。

摘　　要：目的探讨小凹蛋白(Cav)-1、Cav-2在宫颈鳞状细胞癌及鳞状上皮内病变组织中的表达及意义。方法收集2016年1月至2020年12月乌鲁木齐市妇幼保健院病理科的宫颈手术切除石蜡包埋组织样本,包括正常组织40例、宫颈低级别鳞状上皮内病变(LSIL)组织样本40例、宫颈高级别鳞状上皮内病变(HSIL)组织样本40例、宫颈鳞状细胞癌组织样本40例。应用免疫组织化学法检测正常组织、宫颈HSIL组织样本、宫颈LSIL组织样本及宫颈鳞状细胞癌组织样本中Cav-1、Cav-2的表达,应用实时荧光定量聚合酶链反应技术检测宫颈鳞状细胞癌和正常组织中Cav-1、Cav-2及上皮-间充质转化相关分子[表皮生长因子受体(EGFR)、上皮钙黏素(E-cadherin)、神经钙黏素(N-cadherin)及波形蛋白(Vimentin)]的信使RNA表达。结果宫颈鳞状细胞癌组Cav-1评分高于正常组、宫颈LSIL组、宫颈HSIL组[6.00(6.00,8.25)分比0.00(0.00,1.00)分、0.00(0.00,1.00)分、0.00(0.00,0.00)分](P<0.05),宫颈HSIL组与宫颈鳞状细胞癌组Cav-2评分均高于正常组和宫颈LSIL组,且宫颈鳞状细胞癌组Cav-2评分高于宫颈HSIL组[9.00(6.00,9.00)分比2.00(2.00,3.00)分](P<0.05)。宫颈鳞状细胞癌组Cav-1表达低于正常组(P<0.05);正常组与宫颈鳞状细胞癌组Cav-2、EGFR、E-cadherin、N-cadherin及Vimentin表达比较差异无统计学意义(P>0.05)。结论Cav-1、Cav-2在宫颈鳞状细胞癌中的表达明显高于宫颈鳞状上皮内病变及正常组织,证实其参与了宫颈鳞状细胞癌的发生发展;Cav-2在宫颈HSIL中的表达明显高于宫颈LSIL和正常组织,故Cav-2可能作为宫颈鳞状上皮内病变分级的鉴别诊断标志物。Objective To investigate the expression and significance of caveolin(Cav)-1 and Cav-2 in cervical squamous cell carcinoma and squamous intraepithelial lesions.Methods Paraffin-embedded tissue samples were collected from Department of Pathology,Urumqi Maternal and Child Health Hospital from Jan.2016 to Dec.2020,including 40 cases of normal tissue,40 cases of low-grade cervical squamous intraepithelial lesions(LSIL),40 cases of high-grade cervical squamous intraepithelial lesions(HSIL)and 40 cases of cervical squamous cell carcinoma.Immunohistochemistry was used to detect the expression of Cav-1 and Cav-2 in normal tissues,cervical HSIL,cervical LSIL and cervical squamous cell car-cinoma.The messenger RNA levels of Cav-1,Cav-2 and epithelial-mesenchymal transition-related molecules[epidermal growth factor receptor(EGFR),epithelial cadherin(E-cadherin),neuro-cadherin(N-cadherin)and Vimentin]in cervical squamous cell carcinoma and normal tissues were detected by real-time fluorescence quantitative polymerase chain reaction expression.Results The score of Cav-1 in cervical squamous cell carcinoma group was higher than that in the normal group,cervical LSIL group and cervical HSIL group[6.00(6.00,8.25)vs 0.00(0.00,1.00),0.00(0.00,0.00),0.00(0.00,0.00)](P<0.05).The Cav-2 score of the cervical HSIL group and cervical squamous cell carcinoma group was higher than that of the normal group and cervical LSIL group,and the Cav-2 score of the cervical squamous cell carcinoma group was higher than that of the cervical HSIL group[9.00(6.00,9.00)vs 2.00(2.00,3.00)](P<0.05).The expression of Cav-1 in the cervical squamous cell carcinoma group was lower than that in the normal group(P<0.05).There was no statistically significant difference in the expression ratio of Cav-2,EGFR,E-cadherin,N-cadherin and Vimentin between the normal group and the cervical squamous cell carcinoma group(P>0.05).Conclusion The expressions of Cav-1 and Cav-2 in cervical squamous cell carcinoma are significantly higher than those in cervical squamous cell

关 键 词：宫颈鳞状细胞癌 宫颈鳞状上皮内病变 小凹蛋白-1 小凹蛋白-2 上皮-间充质转化 

分 类 号：R737.33[医药卫生—肿瘤]