高危型HPV感染对宫颈阴道微生态、miR-149-3p、CYBRD1表达的影响  

Effects of the high-risk human papillomavirus infection of patients on their cervical and vaginal microecology and the expressions of miR-149-3p and human cytochrome b reductase 1

作　　者：李琼 谢林峻 庞君 陈稀文 LI Qiong;XIE Lijun;PANG Jun;CHEN Xianwen(Chengdu Seventh People's Hospital,Chengdu,Sichuan Province,610044)

机构地区：[1]四川省成都市第七人民医院,610044

出　　处：《中国计划生育学杂志》2024年第9期2141-2145,共5页Chinese Journal of Family Planning

摘　　要：目的:探讨宫颈炎患者高危型HPV(HR-HPV)感染对宫颈阴道微生态、miR-149-3p、CYBRD1表达的影响。方法:选取2020年12月-2022年12月因宫颈炎就诊于本院的102例临床资料,根据是否感染HR-HPV分入高危HPV阴性组(n=65)与阳性组(n=37)。检测HPV阳性患者HPV感染亚型,比较两组阴道微生态改变及阴道微生态平衡状态;采用荧光定量PCR法检测宫颈脱落细胞miR-149-3p、CYBRD1水平;Spearman法分析宫颈阴道微生态与宫颈脱落细胞miR-149-3p、CYBRD1水平的相关性;采用多因素logistic逐步回归分析HR-HPV感染影响因素。结果:HR-HPV阳性组宫颈病变严重程度高于阴性组,阳性组中HPV16亚型检出率最高(51.0%),其次为HPV18(18.6%);阳性组阴道微生态失衡、解脲脲原体阳性率、乳酸杆菌异常率均高于阴性组,miR-149-3p(0.53±0.12)低于阴性组(1.67±0.23),CYBRD1相对表达量(1.29±0.27)高于阴性组(0.46±0.10)(均P<0.05);HR-HPV感染与宫颈脱落细胞miR-149-3p表达呈负相关、与CYBRD1表达、阴道微生态失衡、解脲脲原体阳性率及乳酸杆菌异常率呈正相关(均P<0.05)。HR-HPV阳性组不良孕产史占比高于阴性组(P<0.05)。多因素分析显示:解脲脲原体阳性率、乳酸杆菌异常率、不良孕产史、阴道微生态失衡、miR-149-3p降低、CYBRD1升高是HR-HPV感染影响因素(均P<0.05)。结论:HR-HPV感染者宫颈脱落细胞中miR-149-3p低表达、CYBRD1高表达,亚型检出以HPV16、HPV18为主,且HR-HPV感染会影响患者宫颈阴道微生态失衡,增加宫颈病变严重程度。Objective:To explore the effects of high-risk human papillomavirus(HR-HPV)infection of the patients on their cervical and vaginal microecology and the expressions of miR-149-3p and human cytochrome b reductase 1(CYBRD1)in their cervical exfoliated cells.Methods:The clinical data of 102 patients who were admitted to the hospital due to cervicitis from December 2020 to December 2022 were selected in this study.These women were divided in to group A(65 cases without HPV infection)and group B(37 cases with HPV infection),and the HPV infection subtypes of the patients in group B were detected.The vaginal microecological changes and the vaginal microecological balance of the patients were compared between the two groups.The levels of miR-149-3p and CYBRD1 in the cervical exfoliated cells of the patients in the two groups were detected by fluorescence quantitative PCR.Spearman method was used to analyze the correlation between the cervical vaginal microecology of the patients and their miR-149-3p and CYBRD1 expressions in the cervical exfoliated cells.Multi-factor logistic stepwise regression analysis was used to explore the influence factors of the HR-HPV infection of the patients.Results:The severity of cervical lesions of the patients in group B was significantly higher than that of the patients in group A.In group B,the subtype HPV16 detection rate was the highest(51.0%),and then followed by HPV18(18.6%).The vaginal microecological imbalance,the positive rate of ureaplasma urealyticum and the abnormal rate of lactobacillus of the patients in group B were significantly higher than those of the patients in group A.The expression of miR-149-3p(0.53±0.12)of the patients in group B was significantly lower than that(1.67±0.23)of the patients in group A,and the relative expression of CYBRD1of the patients(1.29±0.27)in group B was significantly higher than that(0.46±0.10)of the patients in group A(all P<0.05).The HR-HPV infection of the patients showed the negative correlation with the miR-149-3Pexpression in their cerv

关 键 词：高危型人乳头瘤病毒 miR-149-3p 人细胞色素b还原酶1 宫颈阴道微生态 相关性 影响因素 

分 类 号：R737.33[医药卫生—肿瘤]