基于网络药理学探究逍遥散和绿茶多酚联合用药对急性肝损伤的预防性保护作用机制  

Exploring the preventive protective mechanism of combined use of Xiaoyao San and green tea polyphenols on acute liver injury based on network pharmacology

作　　者：吴希尧 彭穗扬 刘希蕾 刘俊锋 沈媛 吴文韬 周瑞鸿 冯伟艳 冯思源 陈培琪 林丘瑕 宋词 王茉琳 崔宇 WU Xiyao;PENG Suiyang;LIU Xilei;LIU Junfeng;SHEN Yuan;WU Wentao;ZHOU Ruihong;FENG Weiyan;FENG Siyuan;CHEN Peiqi;LIN Qiuxia;SONG Ci;WANG Molin;CUI Yu(School of Medicine,Sun Yat-sen University,Guangdong,Shenzhen 518107,China)

机构地区：[1]中山大学医学院,广东深圳518107

出　　处：《中国医药科学》2024年第17期26-29,78,共5页China Medicine And Pharmacy

基　　金：2022年广东省本科高校质量工程建设项目(粤教高函〔2023〕4号)。

摘　　要：目的基于网络药理学探究逍遥散和绿茶多酚联合用药对急性肝损伤的预防性保护作用机制。方法基于网络药理学筛选逍遥散、绿茶多酚的化学成分和潜在作用靶点交集,利用GeneCards与NCBI数据库查询与急性肝损伤相关的靶点,在STRING平台进行蛋白质-蛋白质相互作用(PPI)分析,利用DAVID数据库对潜在作用靶点进行京都基因与基因组百科全书(KEGG)通路富集分析与基因本体(GO)分析,预测药物联合作用的机制及通路。结果逍遥散可通过异紫苏黄酮、异白叶醇等潜在关键成分作用于缺氧诱导因子(HIF)-1A靶点,启动HIF-1信号通路,调控炎症反应的发生并发挥抗氧化应激作用;与绿茶多酚合用时增强对HIF-1A靶点的效应,从而协同增强其预防保护功效。结论本研究初步明确了逍遥散通过多成分、多靶点、多通路预防保护急性肝损伤的作用机制,并探明了其与绿茶多酚合用时潜在的协同增强作用及机制,为中医临床联合用药优化给药方案及新型药物开发提供理论依据。Objective To explore the possible mechanism of the preventive protective effect of combined use of Xiaoyao San and green tea polyphenols on acute liver injury based on network pharmacology.Methods The chemical constituents and potential target intersections of Xiaoyao San and green tea polyphenols were screened based on network pharmacology.GeneCards and NCBI database were used to query target associated with acute liver injury.Protein-protein interactions(PPI)analysis was performed on the STRING platform,and the Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis and gene ontology(GO)analysis were performed on potential targets using the DAVID database to predict the mechanism and pathway of drug combination effects.Results Xiaoyao San can act on the target of hypoxiainducible factor(HIF)-1A through its potential key components,such as Isolicoflavonol and Isotrifoliol,start HIF-1 signaling pathway,regulate the occurrence of inflammatory reaction and play an anti-oxidative stress role.When combined with green tea polyphenols,it can enhance the effect on HIF-1A target,thus synergistically enhancing its preventive and protective efficacy.Conclusion This study preliminarily clarified the mechanism of Xiaoyao San in preventing and protecting acute liver injury through multiple components,targets,and pathways,and explored its potential synergistic enhancement effect and mechanism when used in combination with green tea polyphenols,providing a theoretical basis for optimizing the dosage scheme and developing new drugs for clinical combination of traditional Chinese medicine.

关 键 词：网络药理学 逍遥散 绿茶多酚 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]