基于网络药理学和细胞实验探索柳穿鱼黄素对前列腺癌细胞的作用  

Effects of Pectolinarigenin on Prostate Cancer Based on Network Pharmacology and Cellular Experiment

作　　者：阮诗剑 蔡敬 谢孟婷 刘艳[2] 宁志丰[3] 刘复兴[3] 贾爱亭 周红[3] RUAN Shi-jian;JIA Ai-ting;ZHOU Hong(Department of Internal Medicine,The First Affiliated Hospital of Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院附属第一医院内科,湖北咸宁437100 [2]湖北科技学院医学部药学院 [3]湖北科技学院医学部基础医学院

出　　处：《湖北科技学院学报（医学版）》2024年第5期400-404,408,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院省级大学生创新创业项目(S202310927021)。

摘　　要：目的采用网络药理学、分子对接和细胞实验,探索柳穿鱼黄素对前列腺癌细胞潜在靶点和对细胞增殖、迁移、侵袭的影响及作用机制。方法首先通过数据库筛选柳穿鱼黄素的成分靶点以及前列腺癌相关的疾病靶点,以此来构建药物-靶点-疾病可视化网络和蛋白相互作用网络(PPI),进行GO和KEGG富集分析,采用分子对接软件对潜在的作用靶点进行验证。采用不同浓度的柳穿鱼黄素处理前列腺癌细胞,通过MTT测定和平板克隆形成证明柳穿鱼黄素在前列腺癌细胞增殖中的抑制作用,通过划痕实验以及Transwell侵袭迁移实验证明其侵袭迁移能力。结果网络药理学结果显示柳穿鱼黄素抗前列腺癌的靶点共有62个,KEGG富集分析发现柳穿鱼黄素通过癌症通路、PI3K-AKT等多种信号通路发挥抗前列腺癌的作用;分子对接显示柳穿鱼黄素与主要靶点之间具有良好的结合活性;与空白对照组相比,体外细胞实验结果显示柳穿鱼黄素能显著降低前列腺癌细胞活性,并且其增殖、迁移能力显著降低。结论柳穿鱼黄素可能通过多种靶点抑制前列腺癌细胞增殖。经体外细胞实验验证表明,随着柳穿鱼黄素浓度增加,前列腺癌细胞的增殖、侵袭和迁移能力被抑制。Objective To investigate the effects of Pectolinarigenin on prostate cancer cell proliferation,migration,invasion and potential targets based on network pharmacology,molecular docking technique and cellular assays.Methods Databases were searched for Pectolinarigenin component targets as well as disease targets associated with prostate cancer in order to create a drug-target-disease visualization network and protein-protein interaction(PPI)network.GO and KEGG enrichment analyses were then performed,and potential targets were confirmed with molecular docking tools.Prostate cancer cells were treated with different concentrations of Pectolinarigenin,and the inhibition on the proliferation of prostate cancer cells was demonstrated using MTT and plate clone formation assays.Scratch tests and transwell invasion and migration were used to prove its invasive and migratory abilities.Results The results of network pharmacology showed that there were 62 targets for the anti-prostate cancer effect of Pectolinarigenin.KEGG enrichment analysis revealed that Pectolinarigenin exerts its anti-prostate cancer effects through various signaling pathways such as cancer pathways and PI3K-AKT.Molecular docking demonstrated that Pectolinarigenin has good binding activity with major targets.In vitro cell experiments showed that compared with the blank control group,Pectolinarigenin significantly reduced the activity of prostate cancer cells,as well as their proliferation and migration abilities.Conclusion Pectolinarigenin may inhibit the proliferation and migration of prostate cancer cells in a concentration-dependent manner via a variety of pathways.

关 键 词：柳穿鱼黄素 前列腺癌细胞 增殖 网络药理学 分子对接 

分 类 号：R965[医药卫生—药理学]