急性前葡萄膜炎疾病严重程度与辅助性T细胞相关因子水平的关系探讨  

Relationship between severity of acute anteruor uveitis and level of helper T cell related factors

作　　者：葛晓芳 冯会芳 刘亚东 魏星[1] GE Xiao-fang;FENG Hui-fang;LIU Ya-dong;WEI Xing(Department of Ophthalmology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第二附属医院眼科,郑州450000

出　　处：《医药论坛杂志》2024年第14期1469-1472,1479,共5页Journal of Medical Forum

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190323)。

摘　　要：目的探讨急性前葡萄膜炎(acute anterior uveitis,AAU)疾病严重程度与辅助性T细胞(helper T cell,Th)相关细胞因子水平的关系。方法采用前瞻性病例对照研究设计,收集2021年5月至2022年5月在郑州大学第二附属医院眼科就诊的AAU患者40例,另选取同期体检者30例为对照组,采用ELISA检测受检者外周血中白细胞介素-4(IL-4)、白细胞介素-9(IL-9)、白细胞介素-(IL-17)以及转化生长因子-β1(TGF-β1),比较两组上述指标水平的差异性,并根据EIU评分衡量AAU疾病严重情况,分析AAU患者IL-4、IL-17、IL-9和TGF-β1水平与EIU评分的关系。结果AAU组患者的IL-4、IL-9、IL-17和TGF-β1水平均较对照组高(P<0.05)。IL-4、IL-9、IL-17以及TGF-β1均与EIU评分呈正相关(r=0.426、0.544、0.596、0.514,P=0.006、0.000、0.000、0.001)。AAU患者的EIU评分中,0~3分12例,4~6分15例,7~9分13例。IL-4与IL-9呈正相关(r=0.418,P=0.007),IL-4与IL-17、TGF-β1均无明显相关性(r=0.293、0.265,P均>0.05),IL-9与IL-17、TGF-β1均无明显相关性(r=0.129、0.161,P均>0.05),IL-17与TGF-β1无明显相关性(r=0.280,P>0.05)。结论Th相关细胞因子水平变化与AAU的发生发展密切相关,且IL-4与IL-9呈相关性,可能在AAU的发病机制中有着较为重要的作用。Objective To investigate the relationship between the severity of acute anterior uveitis(AAU)and the level of helper T cell(Th)related cytokines.Methods A prospective case-control study design was used.A total of 40 patients with AAU admitted to the ophthalmology department of the Second Affiliated Hospital of Zhengzhou University from May 2021 to May 2022 were collected,and another 30 patients who underwent physical examination during the same period were selected as the control group.The levels of interleukin-4(IL-4),interleukin-9(IL-9),interleukin-17(IL-17)and transforming growth factor-β1(TGF-β1)in peripheral blood of the patients were detected by ELISA,and the differences of the above indexes between the two groups were compared.The severity of AAU disease was measured according to EIU score,and the relationship between the levels of IL-4,IL-17,IL-9 and TGF-β1 and EIU score in AAU patients was analyzed.Results The levels of IL-4,IL-9,IL-17 and TGF-β1 in AAU group were higher than those in control group(P<0.05).IL-4,IL-9,IL-17 and TGF-β1 were positively correlated with EIU score(r=0.426,0.544,0.596,0.514,P=0.006,0.000,0.000,0.001).The EIU score of AAU patients was 0-3 in 12 cases,4-6 in 15 cases,and 7-9 in 13 cases.There was a positive correlation between IL-4 and IL-9(r=0.418,P=0.007),but no significant correlation between IL-4 and IL-17 and TGF-β1(r=0.293,0.265,bothP>0.05).There was no significant correlation between IL-9 and IL-17 and TGF-β1(r=0.129,0.161,both P>0.05),and no significant correlation between IL-17 and TGF-β1(r=0.280,P>0.05).Conclusion The changes of Th-related cytokines are closely related to the occurrence and development of AAU,and IL-4 and IL-9 exhibita correlation that may play an important role in the pathogenesis of AAU.

关 键 词：急性前葡萄膜炎 辅助性T细胞 细胞因子 自身免疫性疾病 

分 类 号：R773[医药卫生—眼科]