膳食铜补充对大鼠心肌梗死后心肌基质金属蛋白酶2表达水平及血流动力学的影响  

Effect of dietary copper supplementation on the expression level of myocardial matrix metalloproteinase 2 and hemodynamics after myocardial infarction in rats

作　　者：刘聪辉 何浩然 黄一诺 张凤 王凡月 郝翰 Liu Conghui;He Haoran;Huang Yinuo;Zhang Feng;Wang Fanyue;Hao Han(Department of Adult Internal Medicine,Hainan Women and Children′s Medical Center,Haikou 570100,China;College of Pediatrics,Hainan Medical University,Haikou 570100,China)

机构地区：[1]海南省妇女儿童医学中心成人内科,海口570100 [2]海南医科大学儿科学院,海口570100

出　　处：《中华诊断学电子杂志》2024年第3期166-172,共7页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：海南省卫生健康行业科研项目(21A200228);海南省临床医学中心建设项目(琼卫医函〔2021〕75号);大学生创新创业训练计划项目(S202311810044)。

摘　　要：目的探讨膳食铜补充对大鼠心肌梗死(MI)后心肌基质金属蛋白酶2(MMP-2)表达水平及血流动力学的影响。方法取成年雄性SD大鼠40只,6~8周龄,体重(260±15)g。采用随机数字表法,分为假手术对照组(对照组,n=10),MI组(n=15)和膳食铜补充组(MI-铜组,n=15)。MI组和MI-铜组进行MI模型制作,对照组进行MI假手术。MI-铜组给予充足铜饮食(20 mg/kg),对照组与MI组给予铜饮食(6 mg/kg)。精心饲养4周后,电生理仪中导出大鼠手术前后ST段偏移值,检测大鼠左心室舒张末压(LVDEP)、左心室收缩压(SYS)、平均动脉压(MBP)、左心室内压最大上升速率(+LVdp/dtmax)与左心室内压最大下降速率(-LVdp/dtmax)。Masson染色镜下观察大鼠MI后病理变化,应用酶联免疫吸附试验检测心肌MMP-2表达水平。采用配对t检验比较手术前后ST段偏移值,采用单因素方差分析及LSD-t检验比较组间血流动力学指标及MMP-2表达水平。结果造模后,对照组成活8只,MI组成活8只,MI-铜组成活10只。MI组+MI-铜组心电ST段偏移值术后[(33.00±3.24)mV]与术前[(2.90±1.14)mV]比较,明显升高(t=-44.303,P<0.01)。MI-铜组LVDEP[(24.10±0.75)mmHg](1 mmHg=0.133 kPa)较MI组[(32.49±1.82)mmHg]明显降低(t=-15.93,P<0.01),MI组与MI-铜组LVDEP均较对照组[(1.41±0.09)mmHg]明显升高(t=55.93,43.03,均P<0.01)。MI-铜组SYS[(75.18±2.33)mmHg]、MBP[(62.54±1.86)mmHg]、+LVdp/dtmax[(2592.00±192.52)mmHg/s]、-LVdp/dtmax[(2188.00±286.15)mmHg/s]均较MI组[(65.20±1.63)mmHg,(55.90±1.66)mmHg、(1722.50±162.28)mmHg/s,(1643.75±101.55)mmHg/s]明显升高(t=6.12,4.41,10.36,5.76;均P<0.01),MI组与MI-铜组SYS、MBP、+LVdp/dtmax、-LVdp/dtmax均较对照组[(112.31±5.42)mmHg,(104.19±5.10)mmHg,(5839.75±170.21)mmHg/s,(4693.75±120.70)mmHg/s]明显降低(t=-27.35,30.40,-46.52,-30.65,-22.72,-27.64,-38.68,-26.54;均P<0.01)。对照组左心室心肌厚度均匀,心室腔正常;MI组和MI-铜组可见左心室梗死区域心肌明显变薄,胶原纤维Masson染色�Objective To explore the effect of dietary copper supplementation on the expression of myocardial matrix metalloproteinase 2(MMP-2)and hemodynamics after myocardial infarction(MI)in rats.Methods Forty adult male Sprague-Dawley rats,aged 6-8 weeks and weighing(260±15)g,were selected.Rats were divided into a sham operation control group(control group,n=10),an MI group(n=15),and a dietary copper supplementation group(MI-Cu group,n=15)using the method of random number table.MI models were made in MI group and MI-Cu group,and control group performed sham MI operation.The MI-Cu group was given an adequate copper diet(20 mg/kg),and the control group and the MI group were given a copper diet(6 mg/kg).After 4 weeks of careful feeding,the pre-and post-operative ST segment deviation values of rats was exported from the electrophysiological instrument.The left ventricular end diastolic pressure(LVDEP),left ventricular systolic pressure(SYS),mean arterial pressure(MBP),maximal rate of increase of left ventricular pressure(+LVdp/dtmax),and maximal rate of decrease of left ventricular pressure(-LVdp/dtmax)were detected.The pathological changes after MI in rats were observed under a microscope after Masson staining.The expression of myocardial MMP-2 in rats was detected by enzyme linked immunosorbent assay.Paired t-test was used to compare the ST segment deviation values before and after surgery.One-way analysis of variance and LSD-t test were used to compare the hemodynamic indexes and MMP-2 expression levels between groups.Results After the model was established,8 rats survived in the control group,8 rats survived in the MI group,and 10 rats survived in the MI-Cu group.The ST segment deviation value of the electrocardiogram in the MI group and MI-Cu group after surgery[(33.00±3.24)mV]was significantly higher than that before surgery[(2.90±1.14)mV](t=-44.303,P<0.01).LVDEP in the MI-Cu group[(24.10±0.75)mmHg,1 mmHg=0.133 kPa]was significantly lower than that in the MI group[(32.49±1.82)mmHg](t=-15.93,P<0.01).LVDEP in the MI

关 键 词：心肌梗死 膳食 铜 基质金属蛋白酶2 血流动力学 

分 类 号：R542.22[医药卫生—心血管疾病]