厚垣镰孢霉固体发酵产葡聚糖内切酶的条件优化  

Optimization of Conditions for Producing Endoglucanase by Solid-state Fermentation of Fusarium chlamydosporum

作　　者：张健[1] ZHANG Jian(Guangxi Health Science College,Nanning,Guangxi 530023,China)

机构地区：[1]广西卫生职业技术学院,广西南宁530023

出　　处：《贵州农业科学》2024年第9期74-79,共6页Guizhou Agricultural Sciences

基　　金：广西自然科学基金项目(2020GXNSFAA297218)。

摘　　要：【目的】优化厚垣镰孢霉固体发酵产葡聚糖内切酶的条件,为纤维素酶应用奠定基础。【方法】以厚垣镰孢霉HML278为研究对象,采用固体发酵方法分析其产葡聚糖内切酶的条件即发酵时间、发酵温度、接种量和培养基料液比对葡聚糖内切酶酶活力的影响,通过正交试验筛选厚垣镰孢霉固态产酶的最优发酵条件。【结果】葡萄糖浓度线性回归方程:y=0.0465x+0.0177,R 2=0.999。单因素试验中,料液比为1︰2时酶活最高,为2.86 U/mL;孢子接种量为孢子悬液1.5 mL/100g物料时酶活最高,为3.29 U/mL;发酵温度为40℃时酶活最高,为3.67 U/mL;发酵时间为4 d时酶活最高,为3.67 U/mL。正交试验显示,最优发酵条件为培养基料液比1︰2、发酵温度40℃、发酵时间5 d,葡聚糖内切酶酶活为4.15 U/mL。【结论】优化的厚垣镰孢霉固体发酵条件产葡聚糖内切酶的酶活较高,降解纤维素效果佳。【Objective】The conditions for producing endoglucanase by solid-state fermentation of Fusarium chlamydosporum were optimized to lay the foundation for the application of cellulase.【Method】The effects of the conditions of fermentation time,fermentation temperature,inoculation amount,and medium solid-liquid ratio for F.chlamydosporum HML278 producing endoglucanase on enzyme activity were analyzed by solid-state fermentation method,and then the optimal fermentation conditions for solid-state enzyme production by F.chlamydosporum were screened through orthogonal experiments.【Result】The linear regression equation of glucose concentration was y=0.0465x+0.0177,R 2=0.999.Under the single factor experiment,the enzyme activity reached the highest with 2.86 U/mL as the solid-liquid ratio was 1︰2,the enzyme activity reached the highest with 3.29 U/mL as the spore inoculation amount was 1.5 mL/100g,the enzyme activity reached the highest with 3.67 U/mL as fermenting at 40℃,and the enzyme activity reached the highest with 3.67 U/mL as fermenting for 4 days.Under the orthogonal experiment,the optimal fermentation conditions included 1︰2 of medium solid-liquid ratio,fermenting at 40℃for 5 days,and endoglucanase activity was 4.15 U/mL.【Conclusion】The optimized conditions for producing endoglucanase by the solid-state fermentation of F.chlamydosporum obtain the higher enzyme activity,and the degradation effect of cellulose is good.

关 键 词：厚垣镰孢霉酶 固体发酵 条件优化 葡聚糖内切酶 培养基 酶活 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]