面向育种应用的高州野生稻片段导入系构建与产量性状QTL分析  

Construction and QTL Analysis of Yield Traits of Gaozhou Wild Rice Chromosome Segment Introgression Lines Targeted at Breeding

作　　者：霍兴 柳武革[1] 刘迪林[1] 廖亦龙[1] 付崇允[1] 朱满山[1] 李金华[1] 曾学勤[1] 马晓智 孔乐 王丰[1] HUO Xing;LIU Wuge;LIU Dilin;LIAO Yilong;FU Chongyun;ZHU Manshan;LI Jinhua;ZENG Xueqin;MA Xiaozhi;KONG Le;WANG Feng(Rice Research institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Genetics and Breeding of High Quality Rice in South China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院水稻研究所/农业农村部华南优质稻遗传育种重点实验室(部省共建)/广东省水稻育种新技术重点实验室/广东省水稻工程实验室,广州510640

出　　处：《中国稻米》2024年第5期62-66,71,共6页China Rice

基　　金：国家重点研发计划项目“农业生物重要性状形成与环境适应性基础研究”(2022YFF1002600);广东省农业科学院科技创新战略专项(高水平农科院建设)(R2018YJ-YB1001)。

摘　　要：野生稻是水稻育种的宝贵基因库,但基因组杂合性高,与栽培稻差异大,为育种上直接利用野生稻带来了困难。构建野生稻片段导入系为挖掘和利用野生稻基因资源提供了有效的技术手段。本研究以广东高州野生稻为供体,粳稻品种中花11为受体,通过多代杂交和自交,以育种目标性状为筛选标准,甄选出100个片段导入系。对这100个导入系的产量相关性状(单株有效穗数、总实粒数、千粒重、每穗实粒数和单株产量)进行考察,发现它们至少有1个性状要优于对照品种中花11,显示出在产量改良上的应用潜力。导入系全基因组覆盖度46.9%,共识别出20个产量相关QTL。这些片段导入系作为高州野生稻新型种质资源,为利用野生稻助力水稻种质创新提供了资源储备。Wild rice is a valuable gene pool for rice breeding,but its high genomic heterozygosity and significant differences from cultivated rice pose challenges for directly utilizing wild rice in breeding.Constructing chromosome segment introgression lines provides an effective technical approach to access and utilize wild rice genetic resources.We selected 100 introgression lines using Gaozhou wild rice as the donor and japonica rice variety Zhonghua 11 as the recipient,through multi-generational hybridization and self-crossing with breeding target traits as the screening criteria and examined the yield traits(number of effective panicles per plant,total filled grains per plant,thousand-grain weight,average filled grain number per panicle,and grain yield per plant)of these 100 lines,found that at least one trait was superior to the control variety Zhonghua 11,demonstrating the potential for yield improvement.The introgression lines had a whole-genome coverage of 46.9%and identified 20 yield-related QTLs.These segment introgression lines represent a new type of germplasm resource derived from Gaozhou wild rice and provide a reserve for promoting innovation in rice germplasm from wild rice.

关 键 词：水稻 野生稻 片段导入系 产量性状 QTLS 

分 类 号：S511.032[农业科学—作物学]