乳腺癌组织中PXMP4的表达及其对乳腺癌细胞生物学行为的影响  

Expression of PXMP4 in breast cancer tissues and its effects on biological behavior of breast cancer cells

作　　者：李巍 张静雨 布海霞 陈庆庆 孙许朋 千新来[2,3] 贺国洋[2,3] LI Wei;ZHANG Jingyu;BU Haixia;CHEN Qingqing;SUN Xupeng;QIAN Xinlai;HE Guoyang(School of Forensic Medicine,Xinxiang Medical University,Xinxiang 453003,China;School of Basic Medicine Sciences,Xinxiang Medical University,Xinxiang 453003,China;Department of Pathology,Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院法医学院,新乡453003 [2]新乡医学院基础医学院,新乡453003 [3]新乡医学院第三附属医院病理科,新乡453003

出　　处：《临床与实验病理学杂志》2024年第9期923-930,共8页Chinese Journal of Clinical and Experimental Pathology

基　　金：河南省高等学校重点科研项目(23B310002);河南省大学生创新创业训练计划(202310472007)。

摘　　要：目的 探讨乳腺癌组织中过氧化物酶体膜蛋白4(peroxisomal membrane protein 4, PXMP4)的表达及其对乳腺癌细胞增殖、侵袭和上皮-间质转化(epithelial-mesenchymal transition, EMT)的影响。方法 采用生物信息学和免疫组化分析乳腺癌组织中PXMP4的表达。在乳腺癌细胞中干扰或过表达PMXP4后,Western blot分析细胞Cyclin D1、E-cadherin、vimentin及N-cadherin的表达。利用CCK-8和平板克隆实验分析乳腺癌细胞的增殖能力。划痕愈合和Transwell分析乳腺癌细胞的迁移和侵袭能力。利用慢病毒构建PXMP4沉默的MCF-7细胞系,裸鼠皮下和尾静脉注射PXMP4沉默的MCF-7细胞,观察皮下瘤和肺转移数量。结果 生物信息学和免疫组化分析显示乳腺癌组织中PXMP4表达水平显著升高(P<0.05),且PXMP4表达高的乳腺癌患者预后不良(P<0.05)。临床病理分析发现PXMP4的表达与肿瘤分级和淋巴结转移相关(P<0.05)。细胞实验显示:干扰PXMP4抑制乳腺癌细胞增殖、侵袭和EMT进程(P<0.05);反之,过表达PXMP4促进乳腺癌细胞增殖、侵袭及EMT进程(P<0.05)。裸鼠体内实验显示:PXMP4沉默后,肺转移瘤数目、皮下肿瘤的大小及组织中Ki67的表达显著降低(P<0.05)。结论 PXMP4的表达与乳腺癌肿瘤分级及淋巴结转移相关,且PXMP4促进乳腺癌细胞增殖、侵袭和EMT进程。Purpose To detect the expression of peroxisomal membrane protein 4(PXMP4)in breast cancer tissues and to explore the effect of PXMP4 on the proliferation,invasion,and epithelial-mesenchymal transition(EMT)of breast cancer cells.Methods Bioinformatics and immunohistochemistry(IHC)were used to detect the expression of PXMP4 in breast cancer tissues.In breast cancer cells,Western blot was used to detect the expression of Cyclin D1,E-cadherin,vimentin and N-cadherin after knockdown and overexpression of PMXP4.The proliferation ability of breast cancer cells was analyzed by CCK-8 and plate cloning assay.Scratch healing and Transwell assay analyzed the migration and invasion ability of breast cancer cells.Lentivirus was used to construct a PXMP4-silenced MCF-7 cell line,and the PXMP4-silenced MCF-7 cells were injected into the subcutaneous or tail vein of mice to observe lung metastasis and the number of subcutaneous tumors.Results Bioinformatics and IHC showed that the expression of PXMP4 in breast cancer tissues was significantly increased(P<0.05),and the prognosis of breast cancer patients with high expression of PXMP4 was poor(P<0.05).The clinicopathological analysis showed that the expression of PXMP4 was correlated with tumor grade and lymph node metastasis(P<0.05).In vitro knockdown of PMXP4 inhibited the proliferation,invasion and EMT process of breast cancer cells(P<0.05).Conversely,overexpression of PXMP4 promoted the proliferation,invasion and EMT process of breast cancer cells(P<0.05).In vivo,the number of lung metastases,the size of subcutaneous tumor,and the expression of Ki67 in tumor tissue were significantly decreased after silenced PXMP4(P<0.05).Conclusion PXMP4 is related to tumor grading and lymph node metastasis.PXMP4 promotes proliferation,invasion and EMT process of breast cancer cells.

关 键 词：乳腺癌 PXMP4 EMT 增殖 侵袭 转移 

分 类 号：R737.9[医药卫生—肿瘤]