TRPS1蛋白在乳腺癌肺转移灶中的诊断应用价值  

Diagnostic value of TRPS1 protein in lung metastasis of breast cancer

作　　者：肖诗维 金苏 岳君秋 方娜 XIAO Shiwei;JIN Su;YUE Junqiu;FANG Na(Department of Pathology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430079,China;Center for Breast Cancer,Hubei Cancer Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430079,China)

机构地区：[1]湖北省肿瘤医院/华中科技大学同济医学院附属湖北肿瘤医院病理科,武汉430079 [2]湖北省肿瘤医院/华中科技大学同济医学院附属湖北肿瘤医院乳腺中心,武汉430079

出　　处：《临床与实验病理学杂志》2024年第9期967-972,共6页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的 分析TRPS1蛋白在乳腺癌和肺癌中的表达情况及差异,探讨其在鉴别乳腺癌肺转移灶和原发性肺癌中的诊断应用价值。方法 收集湖北省肿瘤医院病理科确诊为乳腺癌369例,原发性肺癌57例(手术切除样本23例,活检样本34例),35例乳腺癌肺转移灶(手术切除样本3例,活检样本32例)。采用免疫组化EnVision法检测TRPS1蛋白在所有病例中的表达情况,分析其表达差异。结果 TRPS1在369例乳腺癌中均表达,其中93.2%病例呈弥漫强阳性(≥95%肿瘤细胞核强阳性),6.8%病例肿瘤细胞呈异质性表达,肿瘤类型主要为乳腺伴大汗腺分化的癌及化生性癌。57例原发性肺癌中,28.1%病例TRPS1呈阴性,70.2%病例呈异质性表达,表现为强弱不等的核着色或仅局灶核着色,且在不同组织学类型包括腺癌、鳞状细胞癌和神经内分泌癌中均以异质性表达占优势,另在1例(1.7%)肺腺癌活检样本中观察到TRPS1呈弥漫强阳性;35例乳腺癌肺转移灶包括手术切除样本和活检样本,TRPS1均呈弥漫强阳性。TRPS1在乳腺癌肺转移灶和原发性肺癌中的表达差异具有统计学意义(P<0.001)。结论 TRPS1蛋白是乳腺癌的高敏感和特异性生物标志物,但仍然可表达于其他肿瘤如肺癌等,且TRPS1在各种组织学类型肺癌中呈不同程度表达,其在鉴别乳腺癌肺转移灶和原发性肺癌中具有一定的诊断价值,但尚不能作为单一诊断依据,尤其是肺结节小活检组织中,需联合应用多个生物标志物综合判断肿瘤来源以提高诊断准确性,从而指导后续临床治疗。Purpose To analyze the expression of TRPS1 protein in breast cancer and lung cancer and its difference,and to explore the diagnostic value of TRPS1 protein in differential diagnosis between lung metastasis from breast cancer and primary lung cancer.Methods A total of 369 cases of breast cancer,57 cases of primary lung cancer(23 cases of surgical resection samples,34 cases of biopsy samples),and 35 cases of lung metastases from breast cancer(3 cases of surgical resection samples,32 cases of biopsy samples)were collected.Immunohistochemical EnVision method was used to detect the expression of TRPS1 protein in all cases and to analyze its expression differences.Results TRPS1 protein was expressed in 369 cases of breast cancer,of which 93.2%cases showed diffusely strongly positive expression(≥95%strongly positive expression in tumor cell nucleus),6.8%cases showed heterogeneous expression of tumor cells,and the tumor types were mainly breast cancer with sweat gland differentiation and metaplastic cancer.Among 57 cases of primary lung cancer,28.1%of cases were negative for TRPS1,70.2%of cases showed heterogeneous expression,manifested as varying degrees of nuclear staining or only focal nuclear staining,and heterogeneous expression was dominant in different histological types including adenocarcinoma,squamous cell carcinoma,and neuroendocrine carcinoma.In addition,TRPS1 was observed to be diffusely strongly positive in one(1.7%)lung adenocarcinoma biopsy sample.Among 35 cases of lung metastases from breast cancer,including surgical resection samples and biopsy samples,100%cases showed diffusely strongly positive expression of TRPS1.The expression of TRPS1 in lung metastasis of breast cancer and primary lung cancer was significantly different(P<0.001).Conclusion TRPS1 protein is a highly sensitive and specific biomarker for breast cancer,but it can still be expressed in other tumors such as lung cancer,and it is also observed that TRPS1 is expressed to varying degrees in various histological types of lung cancer,which

关 键 词：乳腺肿瘤 乳腺癌肺转移灶 原发性肺癌 TRPS1 鉴别诊断 

分 类 号：R737.9[医药卫生—肿瘤]