异菝葜皂苷元对MPP+诱导的SH-SY5Y细胞凋亡的影响  

Protective Effect of Smilagenin on MPP+-induced Apoptosis in SH-SY5Y Cells

作　　者：范蕾 何萱 茅洪维 张评奥 张永芳[1] FAN Lei;HE Xuan;MAO Hong-wei;ZHANG Ping-ao;ZHANG Yong-fang(Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China;Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,Shanghai,200003,China)

机构地区：[1]上海交通大学医学院,上海200025 [2]上海交通大学医学院附属瑞金医院,上海200003

出　　处：《现代生物医学进展》2024年第14期2601-2605,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(82003719)。

摘　　要：目的:探讨菝葜皂苷元对MPP+诱导的SH-SY5Y细胞凋亡的影响及机制。方法:采用MPP+处理人神经母细胞瘤SH-SY5Y细胞构建体外PD细胞模型。取对数生长期的SH-SY5Y细胞分为四组:Control组(正常对照组),MPP+组(模型组),异菝葜皂苷元组(10μM),MPP++异菝葜皂苷元组(3 m M,10μM)。首先采用细胞增殖实验(CCK-8)检测异菝葜皂苷元作用的最佳浓度及对SH-SY5Y细胞活力的影响。采用AnnexinV-FITC/PI双染法检测异菝葜皂苷元对SH-SY5Y细胞凋亡率的影响,采用蛋白免疫印迹实验检测凋亡相关蛋白Bax,Bcl-2的蛋白表达。结果:细胞活力实验确定10μM/m L的异菝葜皂苷元作为后续试验的添加浓度。与对照组相比,MPP+组细胞活力显著降低(P<0.001),细胞凋亡率显著升高(P<0.001),凋亡相关蛋白Bax/Bcl-2表达显著升高(P<0.01)。相较于MPP+组,MPP++异菝葜皂苷元组细胞活力显著升高(P<0.001),细胞凋亡率显著降低(P<0.01),凋亡相关蛋白Bax/Bcl-2表达显著升高降低(P<0.01)。结论:异菝葜皂苷元可以抑制MPP+诱导的SH-SY5Y细胞凋亡,可能与其调节抗凋亡相关蛋白Bax,Bcl-2的蛋白表达有关。Objective:The purpose of this article was to investigate the exerting protective effect of smilagenin on MPP+-induced apoptosis in SH-SY5Y cells.Methods:In the experiment,PD cell model was constructed using MPP+treated human neuroblastoma SH-SY5Y cells.SH-SY5Y cells in logarithmic growth phase were taken and divided into four groups:the Control group(Control),the MPP+group(model group),the smilagenin group(10μM),and the MPP++smilagenin group(3 mm,10μM).The cell proliferation assay(CCK-8)was firstly used to detect the optimal concentration of smilagenin action and the effect on SH-SY5Y cell viability.The effect of smilagenin on the apoptosis rate of SH-SY5Y cells was detected by AnnexinV-FITC/PI double staining assay,and the protein expression of apoptosis-related proteins Bax,Bcl-2 was detected by protein immunoblotting assay.Results:The cell viability assay determined10μM/m L of smilagenin as the added concentration for the subsequent experiments.Compared with the control group,the MPP+group showed significantly lower cell viability(P<0.001),higher apoptosis rate(P<0.001),and significantly higher expression of apoptosis-related protein Bax/Bcl-2(P<0.01).Compared with the MPP+group,cell viability was significantly higher in the MPP++smilagenin group(P<0.001),apoptosis rate was significantly lower(P<0.01),apoptosis-related protein Bax/Bcl-2 expression was significantly lower(P<0.01).Conclusions:Smilagenin can inhibit MPP+-induced apoptosis in SH-SY5Y cells,which may be related to its regulation of protein expression of apoptosis-related proteins Bax and Bcl-2.

关 键 词：异菝葜皂苷元 中草药 SH-SY5Y 细胞凋亡 帕金森病 

分 类 号：R-33[医药卫生] R742.5R285.5