N-MYC及NDRG1在胃癌组织中的表达及对胃癌细胞生物学特性的影响  

Expression of N-MYC and NDRG1 in gastric cancer tissues and their effects on biological characteristics of gastric cancer cells

作　　者：曲艺琳 章诗伟 秦攀 吉洪亮 李顺清 杨楷 QU Yilin;ZHANG Shiwei;QIN Pan;JI Hongliang;LI Shunqing;YANG Kai(Department of Clinical Laboratory,Hubei Integrated Traditional Chinese and Western Medicine Hospital,Wuhan,Hubei 420000,China;Department of Pathology,Hubei Integrated Traditional Chinese and Western Medicine Hospital,Wuhan,Hubei 420000,China)

机构地区：[1]湖北省中西医结合医院检验科,湖北武汉420000 [2]湖北省中西医结合医院病理科,湖北武汉420000

出　　处：《国际检验医学杂志》2024年第18期2229-2233,2239,共6页International Journal of Laboratory Medicine

基　　金：湖北省卫生健康委员会项目(WJ2021M199)。

摘　　要：目的分析N-MYC及N-MYC下游调节基因1(NDRG1)在胃癌组织中的表达及对胃癌细胞生物学特性的影响。方法收集2021年1月至2023年5月于该院行手术切除且经病理确诊为胃癌的82例患者的胃癌组织及癌旁正常组织,采用实时荧光定量PCR(qPCR)检测N-MYC、NDRG1 mRNA相对表达量,收集患者临床资料,分析N-MYC、NDRG1 mRNA表达与患者临床病理特征关系。选择对数生长期NCI-N87细胞,将N-MYC干扰质粒(si-N-MYC)与其阴性对照(si-NC)分别转染到NCI-N87细胞中,记为si-NC组、si-N-MYC组;将si-N-MYC分别与anti-NC、anti-NDRG1共转染至NCI-N87细胞中,记为si-N-MYC+anti-NC组、si-N-MYC+anti-NDRG1组。采用CCK-8实验检测细胞增殖活性,Transwell侵袭实验检测细胞侵袭能力,Western blotting法检测细胞中N-MYC、NDRG1蛋白表达。结果胃癌组织N-MYC mRNA相对表达量高于癌旁组织(P<0.05),NDRG1 mRNA相对表达量低于癌旁组织(P<0.05)。不同胃癌TNM分期、淋巴结转移、远处转移患者N-MYC、NDRG1 mRNA表达差异有统计学意义(P<0.05)。与si-NC组比较,si-N-MYC组细胞增殖和侵袭能力下降(P<0.05),NDRG1蛋白表达下调(P<0.05)。与si-N-MYC+anti-NC组比较,si-N-MYC+anti-NDRG1组细胞增殖、侵袭能力增加(P<0.05)。N-MYC可靶向调控NDRG1,敲低NDRG1可逆转N-MYC对细胞产生的生物学作用。结论胃癌组织N-MYC mRNA表达上调、NDRG1 mRNA表达下调,二者参与胃癌的发生发展过程,并对胃癌细胞增殖、侵袭等恶性生物学行为有重要调控作用。Objective To analyze the expression of N-MYC and N-MYC downstream regulated gene-1(NDRG1)in gastric cancer tissues,and to assess their effects on biological characteristics of gastric cancer cells.Methods Paired of gastric cancer tissues and adjacent normal tissues resected from 82 cases of pathologically confirmed gastric cancer who underwent surgical treatment in the hospital from January 2021 to May 2023 were collected.Gastric cancer tissues and adjacent normal tissues of 82 patients who were surgically resected and pathologically diagnosed with gastric cancer in the hospital from January 2021 to May 2023 were collected.Real-time quantitative PCR(qPCR)was used to detect the relative mRNA expression levels of N-MYC and NDRG1,and clinical data of the patients were collected.The correlation between the mRNA expression of N-MYC and NDRG1 and clinicopathological features of the patients was discussed.NCI-N87 cells in logarithmic growth phase were selected and cultured in vitro.N-MYC interference plasmid(si-N-MYC)and its negative control(si-NC)was transfected into NCI-N87 cells,respectively,which were recorded as si-NC group and si-N-MYC group.Moreover,si-N-MYC was co-transfected into NCI-N87 cells with anti-NC and anti-NDRG1,respectively,and denoted as si-N-MYC+anti-NC group and si-N-MYC+anti-NDRG1 group.CCK-8 assay was used to detect cell proliferation activity,Transwell assay was used to detect cell invasion ability,and Western blotting assay was used to detect N-MYC and NDRG1 protein expression in cells.Results The relative expression of N-MYC mRNA in gastric cancer tissues was higher than that in paracancer tissues(P<0.05),and the relative expression of NDRG1 mRNA was lower than that in paracancer tissues(P<0.05).There were significant differences in the expression of N-MYC and NDRG1 mRNA in patients with different TNM stages,lymph node metastasis and distant metastasis(P<0.05).Compared with the si-NC group,the cell proliferation and invasion ability of the si-N-MYC group were decreased(P<0.05),and the expressi

关 键 词：胃癌 N-MYC蛋白 N-MYC蛋白下游调节基因1 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]