黄精多糖对低压低氧暴露大鼠心肌能量代谢的改善作用  

IMPROVEMENT OF MYOCARDIAL ENERGY METABOLISM IN HYPOBARIC HYPOXIA EXPOSED RATS BY POLYGONATUM SIBIRICUM POLYSACCHARIDES

作　　者：宋晓娜 王光瑞 张岭 蒲玲玲 陈照立 刘伟丽 SONG Xiao-na;WANG Guang-rui;ZHANG Ling;PU Ling-ling;CHEN Zhao-li;LIU Wei-li(Institute of Environmental and Operational Medicine,Academy of Military Medical Science,Academy of Military Sciences,Tianjin 300050,China)

机构地区：[1]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050

出　　处：《营养学报》2024年第3期270-275,共6页Acta Nutrimenta Sinica

摘　　要：目的探讨黄精多糖(Polygonatum sibiricum polysaccharide,PSP)对低压低氧大鼠心肌能量代谢的改善作用并探讨其机制。方法将24只Wistar大鼠随机分为对照组(control)、低压低氧组(HH)和低压低氧+PSP组(HH+PSP),每组8只。HH组和HH+PSP组大鼠在低压氧舱模拟海拔6000 m进行慢性低压低氧干预2 w,HH+PSP组灌胃给予PSP 400 mg/kg,其余两组灌胃给予等体积生理盐水。每周检测2次体重、2次摄食量,处死前超声心动图检测各组大鼠心功能指标,测定大鼠血清及心肌组织中游离脂肪酸(free fatty acids,FFA)、葡萄糖(glucose,GLU)、乙酰辅酶A(acetyl coenzyme A,A-CoA)以及三磷酸腺苷(adenosine triphosphate,ATP)含量评价能量代谢情况,Western blotting检测大鼠心肌组织过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptor alpha,PPARα)、肉碱棕榈酰转移酶1(carnitine palmitoyltransferase 1,CPT-1)、丙酮酸脱氢酶4(pyruvate dehydrogenase kinase 4,PDK4)、葡萄糖转运蛋白4(glucosetransporter,GLUT4)、甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphatedehydrogenase,GAPDH)的表达水平。结果与对照组相比,HH组大鼠体重和摄食量显著降低(P<0.05);超声心动图显示心输出量、心室末期容积及每搏输出量均降低(P<0.05),心室后壁厚度显著增高(P<0.05);能量代谢相关指标检测显示GLU、ATP显著降低(P<0.05),FFA、A-CoA含量显著升高(P<0.05);Westernblotting结果显示心肌组织PPARα、CPT-1、PDK4表达水平显著降低(P<0.05),GLUT4、GAPDH的表达显著升高(P<0.05)。与HH组相比,HH+PSP组大鼠体重及摄食量无显著变化(P>0.05);心输出量、心室末期容积、心室后壁厚度及每搏输出量均得到显著改善(P<0.05);GLU、ATP含量显著升高(P<0.05),FFA、A-CoA含量显著降低(P<0.05)。此外,PPARα、CPT-1、PDK4、GLUT4、GAPDH的蛋白表达水平也得到显著下调(P<0.05)。结论低压低氧暴露导致大鼠心功能损伤与能量代谢紊乱密切相关,PSP干Objective To investigate the regulatory effect of Polygonatum sibiricum polysaccharides(PSP)on myocardial energy metabolism in hypobaric hypoxia exposed rats and explore its mechanism.Methods Twenty-four Wistar rats were randomly divided into control group(control),hypobaric hypoxia group(HH)and hypobaric hypoxia+PSP group(HH+PSP)with eight rats in each group.Rats in the HH and HH+PSP groups were subjected to chronic HH exposure for 2weeks in a hypobaric chamber at a simulated altitude of 6000m.The HH+PSP group was given 400mg/kg PSP and the other two groups were given equal volume of saline.The body weight and food intake were measured twice a week,and cardiac function was measured by echocardiography at the end of the experiment.Determination of free fatty acids(FFA),glucose(GLU),acetyl coenzyme A(A-CoA)and adenosine triphosphate(ATP)in serum and myocardial tissues was carried out to evaluate energy metabolism.The expression levels of peroxisome proliferator activated receptor alpha(PPARα),carnitine palmitoyltransferase 1(CPT-1),pyruvate dehydrogenase kinase 4(PDK4),glucose transporter(GLUT4)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH)in rat myocardial tissue were examined by Western blotting.Results Compared with control group,rats in the HH group had significantly lower body weight and food intake(P<0.05).Echocardiography showed lower cardiac output,ventricular end volume and stroke volume(P<0.05),while ventricular posterior wall thickness was significantly higher.Energy metabolism-related indexes showed significantly lower GLU and ATP(P<0.05),and significantly higher FFA and A-CoA contents(P<0.05).Western blotting of myocardial tissue proteins showed significantly decreased expression levels of PPARα,CPT-1,PDK4(P<0.05)and significantly increased expression levels of GLUT4 and GAPDH(P<0.05).Compared with the HH group,there were no significant changes in body weight and food intake in the HH+PSP group(P>0.05).Cardiac output,ventricular end volume and stroke volume were improved(P<0.05),and ventricular

关 键 词：低压低氧 黄精多糖 心肌损伤 能量代谢 

分 类 号：R151.2[医药卫生—营养与食品卫生学]