柑橘橘络提取物对低氧小鼠记忆损伤的保护作用  

PROTECTIVE EFFECTS OF CITRUS TANGERINE PITH EXTRACT ON MEMORY IMPAIRMENT IN HYPOXIC MICE

作　　者：杜金秋 李晓艺 虞立霞 姚站馨 王锋 刘颖 DU Jin-qiu;LI Xiao-yi;YU Li-xia;YAO Zhan-xin;WANG Feng;LIU Ying(School of Public Health,Inner Mongolia Medical University,Huhhot O10ll0;Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]内蒙古医科大学公共卫生学院,呼和浩特010110 [2]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050 [3]天津中医药大学,天津301617

出　　处：《营养学报》2024年第3期283-288,共6页Acta Nutrimenta Sinica

基　　金：BLJ22C017

摘　　要：目的探讨柑橘橘络提取物对低氧小鼠记忆损伤的保护作用。方法30只雄性BALB/c小鼠,随机分为常氧对照组(CON)、低氧模型组(HYP)和柑橘橘络提取物组(CIT),每组10只,将后2组小鼠置于模拟海拔6000 m的低压氧舱内,每天8 h,持续28 d。CON组和HYP组灌胃给予0.4 ml纯净水,CIT组灌胃给予15 mg/(kg·bw)柑橘橘络提取物。采用穿梭和跳台实验评价小鼠的主动学习记忆和被动学习记忆能力;采用苏木精-伊红(hematoxylin-eosin,HE)染色观察海马病理学变化;采用生物化学方法检测脑组织乳酸脱氢酶(lacticdehydrogenase,LDH)和ATP酶活力;采用代谢组学方法靶向检测小鼠脑组织糖酵解和三羧酸循环代谢产物。结果与CON组相比,HYP组小鼠的主动穿梭次数减少(P<0.05)、无响应次数增加(P<0.05);跳台实验错误次数增加(P<0.05)、潜伏期缩短(P<0.05);海马细胞排列松散、核固缩增加;ATP、磷酸烯醇式丙酮酸、乌头酸、异柠檬酸、α-酮戊二酸、琥珀酸和草酰乙酸含量显著降低(P<0.05),乳酸、6-磷酸葡萄糖、β-6-磷酸果糖和1,6-二磷酸果糖含量显著升高(P<0.05);LDH和ATP酶活力显著降低(P<0.05)。与HYP组比较,柑橘橘络提取物可以增加穿梭实验主动穿梭次数(P<0.05),降低无响应次数(P<0.05);减少跳台实验错误次数(P<0.05),延长跳下平台的潜伏期(P<0.05);改善海马细胞细胞形态及排列紧密程度;升高ATP和磷酸烯醇式丙酮酸含量(P<0.05),降低乳酸、6-磷酸葡萄糖、β-6-磷酸果糖和1,6-二磷酸果糖含量(P<0.05);降低LDH活力(P<0.05),增强ATPase活力(P<0.05)。结论柑橘橘络提取物具有改善低氧小鼠记忆损伤的作用,推测可能与调控脑能量代谢相关。Objective To explore the protective effects of citrus tangerine pith extract on memory impairment in hypoxic mice.Methods Thirty male BALB/c mice were randomised into normoxia control(CON),hypoxia model(HYP)and citrus tangerine pith extract(CIT)groups,with 10 mice in each group.The latter two groups were placed in a low-pressure oxygen chambers at an altitude simulating 6,000 meters for 8 hours per day for 28 days.The CON group and the HPY group were given 0.4 ml of pure water by gavage,and the CIT group was given 15 mg/(kg·bw)of citrus tangerine pith extract.Shuttle experiment and step-down test were used to assess the active and passive learning and memory abilities.Hematoxylin-eosin staining was used to observe the morphological changes in hippocampus tissue.Biochemical methods were used to determine LDH and ATPase activity in brain tissue.The metabolites of the glycolytic and tricarboxylic acid cycles in brain tissue were identified using metabonomics.Results Compared with the CON group,mice in the HYP group showed a looser arrangement of neurons in the hippocampus and an increase in the nuclear consolidation.In the shuttle experiment,the number of active shuttles was significantly decreased(P<0.05),and the number of non-responses was significantly increased(P<0.05).In the step-down test,the number of errors was significantly increased(P<0.05)and the latency was significantly shortened(P<0.05).The contents of ATP,PEP,aconitrate,isocitrate,KGA,SA,and OA were decreased(P<0.05),while the contents of LA,D-G6P,β-D-F6P and F1,6P were increased(P<0.05).The LDH and ATPase activities of brain tissues in the HYP group were significantly lower(P<0.05).Compared with the HYP group,citrus tangerine pith extract significantly elevated the number of active shuttles in the shuttle experiment(P<0.05)and decreased the number of non-responses(P<0.05).In the step-down test,the number of errors was significantly reduced(P<0.05)and the latency was significantly prolonged(P<0.05).Citrus tangerine pith extract improved the cell mor

关 键 词：低氧 柑橘橘络提取物 小鼠 记忆损伤 能量代谢 

分 类 号：R151.2[医药卫生—营养与食品卫生学]