Tongqiao Yizhi granule(通窍益智颗粒) repress the nuclear factor kappa-b/nucleotide oligomerization domain-like receptors 3/caspase-1 pyroptosis pathway in the hippocampus to counter vascular dementia in rats  

作　　者：LI Yanjiao HAO Linyao LI Shuangyang LUO Yanqi WANG Juan WANG Raoqiong BAI Xue 

机构地区：[1]College of Integrative Medicine,Southwest Medical University,Luzhou 646600,China [2]Department of Neurology and National Traditional Chinese Medicine Clinical Research Base,the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou 646000,China [3]Institute of Integrated Chinese and Western Medicine,Southwest Medical University,Luzhou 646000,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第4期680-687,共8页中医杂志（英文版）

基　　金：Sichuan Provincial Science and Technology Department Applied Basic Research Project:a Dynamic Contrast Enhanced Magnetic Resonance Imaging-based Study on the Effect of Eind Medicine Kaixuan Method on Blood-brain Barrier Function and Prognosis in Patients with Small to Medium Volume Cerebral Hemorrhage(2020YJ0437);Sichuan Provincial Administration of Traditional Chinese Medicine Science and Technology Special:Study on the Mechanism of Shexiang Huayu Xingnao Granules Reducing Nerve Injury in Cerebral Hemorrhage Model Rats by Regulating Microglia Polarization through Peroxisome Proliferator-activated Receptorγ(2021MS506);Luzhou Basic Application Project:the Mechanism of Tongqiao Yizhi Granules in the Treatment of Vascular Dementia by Regulating Gasdermin D-Mediated Pyroptosis(2020-JYJ-57)。

摘　　要：OBJECTIVE:To explore the mechanism by which Tongqiao Yizhi granule(通窍益智颗粒,TQYZKL)intervenes pyroptosis to treat vascular dementia(VaD)in a rat model.METHODS:The rat model of VaD was established by two-vessel occlusion(2VO).The rats were randomly divided into Sham group,Model group,Nimodipine group,TQYZKL(6.2 g·kg^(-1)·d^(-1)),TQYZKL(12.4 g·kg^(-1)·d^(-1)),TQYZKL(24.8 g·kg^(-1)·d^(-1)).The Morris water maze(MWM)test was carried out to test the learning and memory function;Hematoxylin-eosin staining and transmission electron microscopy(TEM)to observe the pathological damage in the hippocampus;Tunel fluorescence staining to detect neuronal pyroptosis in the hippocampus.The expression levels of pyroptosis-related proteins,namely Golgi peripheral membrane protein p65(P65),nucleotide oligomerization domain-like receptors 3(NLRP3),caspase-1 and Gasdermin D(GSDMD),were detected using Western blotting and reverse transcription polymerase chain reaction.Moreover,the serum levels of interleukin-1β(IL-1β)and interleukin-18(IL-18)were determined through the enzyme-linked immunosorbent assay.RESULTS:The study revealed that TQYZKL effectively improved the ability of VaD ratsto learn and memorize,relieved the pathological damage in the hippocampus,restored neuronal morphology,and reduced the expression of pyroptosis-related proteins P65,NLRP3,caspase-1,GSDMD-N,IL-18 and IL-1β(P<0.05).CONCLUSION:TQYZKL inhibits neuronal pyroptosis in the hippocampus of VaD rats by regulating nuclear factor kappa-B/NLRP3/caspase-1 signaling pathway,thus exerting a therapeutic effect on VaD in the rats.

关 键 词：NLRP3 RATS KAPPA 

分 类 号：R285.5[医药卫生—中药学]