An ethically guided preclinical device for phenotyping H_(2)production in laboratory rodents  

作　　者：Victor Pascal-Moussellard Emilie Boucher Stéphane Tanguy Philippe Cinquin Pierre-Alain Barraud ChloéDavin Cordélia Salomez-Ihl Dalil Hannani François Boucher Jean-Pierre Alcaraz 

机构地区：[1]University Grenoble Alpes,CNRS,UMR 5525,VetAgro Sup,Grenoble INP,TIMC,Grenoble,France

出　　处：《Animal Models and Experimental Medicine》2024年第4期553-561,共9页动物模型与实验医学（英文）

基　　金：UniversitéGrenoble Alpes through the Cross Disciplinary Program(CDTools)“My Health Companions”.

摘　　要：Background:Dihydrogen(H_(2))is produced endogenously by the intestinal microbiota through the fermentation of diet carbohydrates.Over the past few years,numer-ous studies have demonstrated the significant therapeutic potential of H_(2)in various pathophysiological contexts,making the characterization of its production in labora-tory species of major preclinical importance.Methods:This study proposes an innovative solution to accurately monitor H_(2)pro-duction in free-moving rodents while respecting animal welfare standards.The devel-oped device consisted of a wire rodent cage placed inside an airtight chamber in which the air quality was maintained,and the H_(2)concentration was continuously analyzed.After the airtightness and efficiency of the systems used to control and maintain air quality in the chamber were checked,tests were carried out on rats and mice with different metabolic phenotypes,over 12 min to 1-h experiments and repeatedly.H_(2)production rates(HPR)were obtained using an easy calculation algorithm based on a first-order moving average.Results:HPR in hyperphagic Zucker rats was found to be twice as high as in control Wistar rats,respectively,2.64 and 1.27 nmol.s^(−1)per animal.In addition,the ingestion of inulin,a dietary fiber,stimulated H_(2)production in mice.HPRs were 0.46 nmol.s^(−1)for animals under control diet and 1.99 nmol.s^(−1)for animals under inulin diet.Conclusions:The proposed device coupled with our algorithm enables fine analysis of the metabolic phenotype of laboratory rats or mice with regard to their endogenous H_(2)production.

关 键 词：hydrogen therapy laboratory rodents MICROBIOTA molecular hydrogen noninvasive monitoring device 

分 类 号：R-332[医药卫生]